Kimura A
J Bacteriol. 1976 Oct;128(1):123-9. doi: 10.1128/jb.128.1.123-129.1976.
The effects on the stringent control of ribosomal ribonculeic acid synthesis of the removal of cell wall, cold-shock treatment of cells, LiCl treatment of toluene-treated cells, and hypotonic treatment of spheroplasts were examined using Escherichia coli rel+ cells. Neither the removal of cell wall with penicillin or lysozyme nor the cold-shock treatment of the cells had an effect on the stringent control. The control mechanism, however, disappeared after the LiCl treatment of the toluene-treated cells, with the release of some protein component(s), possibly from the cytoplasmic membrane. The hypotonic and other treatments of spheroplasts, which disrupt the cytoplasmic membrane, also led to the abolishment of the control mechanism. These results suggested that the operation of the stringent control of ribosomal ribonucleic acid synthesis requires the cytoplasmic membrane, in which some proteins labile with LiCl treatment are embedded.
利用大肠杆菌rel+细胞，研究了细胞壁去除、细胞冷休克处理、用LiCl处理经甲苯处理的细胞以及对原生质体进行低渗处理对核糖体核糖核酸合成严格控制的影响。用青霉素或溶菌酶去除细胞壁以及对细胞进行冷休克处理，均对严格控制没有影响。然而，在用LiCl处理经甲苯处理的细胞后，随着一些可能来自细胞质膜的蛋白质成分的释放，控制机制消失了。对原生质体进行低渗处理和其他破坏细胞质膜的处理，也导致控制机制的废除。这些结果表明，核糖体核糖核酸合成严格控制的运行需要细胞质膜，其中嵌入了一些对LiCl处理敏感的蛋白质。
