Jørgensen J
Acta Endocrinol (Copenh). 1979 Dec;92(4):731-45.
The marked renin inactivation seen during in vitro incubation of post-partum uterine slices which mimics the in vivo condition, is not accompanied by a similar general proteolysis. The inactivating mechanism is so far non-specific with respect to organ or species as added hog renal renin is inactivated at a similar rate as endogenous renin. Endogenous alkaline phosphatase is not significantly inactivated and added alkaline phosphatase is completely stable. A marked inactivation of endogenous renin also takes place during incubation of a mixed mitochondrial-lysosomal suspension prepared from post-partum uterus. The process is more pronounced at pH 7.4 than at 6.8. Freezing and thawing and addition of Triton X-100 prior to incubation inhibits the inactivation. ATP and alpha-ketoglutarate slightly stimulates the process while CoA and chloroquine have no effect. Both iodoacetate and phenylmethylsulphonylfluoride inhibit the inactivation, suggesting that more than one enzyme is involved in the inactivation.
在模仿体内情况的产后子宫切片体外孵育过程中观察到的显著肾素失活，并未伴随着类似的普遍蛋白水解。就器官或物种而言，目前这种失活机制是非特异性的，因为添加的猪肾肾素与内源性肾素以相似的速率失活。内源性碱性磷酸酶没有明显失活，而添加的碱性磷酸酶则完全稳定。在由产后子宫制备的线粒体 - 溶酶体混合悬浮液孵育过程中，内源性肾素也会发生显著失活。该过程在pH 7.4时比在pH 6.8时更为明显。孵育前的冻融和添加 Triton X - 100会抑制失活。ATP和α - 酮戊二酸会轻微刺激该过程，而辅酶A和氯喹则没有作用。碘乙酸和苯甲基磺酰氟都抑制失活，这表明不止一种酶参与了失活过程。
