Jørgensen J
Acta Endocrinol (Copenh). 1981 Feb;96(2):281-8.
A pronounced formation of renin occurs during incubation of non-pregnant uterine tissue slices in vitro. The synthesized renin appears in an enzymatically inactive form, which can be activated by acidification. Prior to incubation only a small fraction of inactive renin is present. The formation of inactive renin is blocked by puromycin and by inhibition of energy metabolism, indicating a de novo synthesis. A similar pattern of inhibition prevails the modest formation of inactive renin in post-partum uterus. The marked loss of active renin seen during incubation of post-partum uterine tissue is partly prevented by an inhibition of energy metabolism. Potent inhibitors are iodoacetate and chloroquine. These findings are in accordance with lysosomal engagement in the inactivation of renin. Incubated kidney cortex tissue shows only a minor loss of renin during incubation. This loss is uninfluenced by attempts to block it.
在非妊娠子宫组织切片的体外孵育过程中会出现明显的肾素形成。合成的肾素以酶无活性形式出现，可通过酸化激活。孵育前仅存在一小部分无活性肾素。无活性肾素的形成被嘌呤霉素和能量代谢抑制所阻断，表明是重新合成。类似的抑制模式在产后子宫中无活性肾素的适度形成中也存在。产后子宫组织孵育期间活性肾素的显著损失部分被能量代谢抑制所阻止。有效的抑制剂是碘乙酸盐和氯喹。这些发现与溶酶体参与肾素失活一致。孵育的肾皮质组织在孵育期间仅显示出少量肾素损失。这种损失不受阻断尝试的影响。
