Raveché E S, Tjio J H, Steinberg A D
Cytogenet Cell Genet. 1979;23(3):182-93. doi: 10.1159/000131324.
The presence of hyperdiploidy was studied in New Zealand black (NZB) mice and the progeny of NZB X DBA/2 crosses and backcrosses. Hyperdiploidy was observed in the spleens of a majority of NZB mice but not in DBA/2 mice at 1 year of age. In crosses of NZB with the DBA/2 strain, hyperploidy was observed only in backcrosses to NZB. Hyperdiploidy appeared to be determined by a recessivley inherited trait and was not related to the presence of other immunological abnormalities, including splenomegaly, hypergammaglobulinemia, and spontaneous antibodies cytotoxic for T cells and reactive with single-stranded DNA. Abnormal cells were not present in Concanavalin A-stimulated 48-h spleen cultures. There was no difference in the in vitro sister chromatid exchange rate between the autoimmune NZB strain and the non-autoimmune DBA/2 strain. Identification of NZB chromosomes by banding analysis showed that chromosomes 15 and 17 were frequently present in more than two copies in hyperdiploid spleen cells. NZB chromsomes also had reduced C-banding in an autosomal pair. These studies indicate that chromosomal abnormalities which occur in NZB mice may be useful as genetic and cytogenetic markers.
对新西兰黑鼠（NZB）及其与DBA/2杂交和回交后代中的超二倍体现象进行了研究。在大多数1岁的NZB小鼠脾脏中观察到超二倍体现象，但在DBA/2小鼠中未观察到。在NZB与DBA/2品系的杂交中，仅在回交到NZB的后代中观察到超倍体现象。超二倍体似乎由隐性遗传性状决定，且与其他免疫异常无关，包括脾肿大、高球蛋白血症以及对T细胞具有细胞毒性且与单链DNA反应的自发抗体。在伴刀豆球蛋白A刺激的48小时脾脏培养物中不存在异常细胞。自身免疫性NZB品系和非自身免疫性DBA/2品系之间的体外姐妹染色单体交换率没有差异。通过染色体显带分析对NZB染色体进行鉴定表明，在超二倍体脾脏细胞中，15号和17号染色体经常出现多于两个拷贝。在一对常染色体中，NZB染色体的C带也减少。这些研究表明，NZB小鼠中出现的染色体异常可能作为遗传和细胞遗传学标记物。
