Case M E, Giles N H
Genetics. 1974 Apr;76(4):715-21. doi: 10.1093/genetics/76.4.715.
Genetical and biochemical studies have been performed with revertants induced in a polyaromatic mutant (No. 58) in the arom gene cluster of Neurospora crassa. In addition to complete and partial revertants able to grow on minimal at both 25 degrees and 35 degrees , temperature-sensitive revertants capable of growth on minimal at 25 degrees but not at 35 degrees have been recovered. One of these revertants has been shown to lack biosynthetic dehydroquinase activity at both temperatures (utilizing the inducible catabolic isozyme for growth at 25 degrees ), to have dehydroshikimate reductase activity only at 25 degrees , and to form an arom aggregate having a molecular weight approximately one-half that of wild type. These results are interpreted as indicating that pleiotropic mutants in the arom gene cluster can result from missense mutations, as well as from nonsense mutations as indicated in previous studies.
对粗糙脉孢菌芳香族基因簇中一个多芳香族突变体（58号）诱导产生的回复突变体进行了遗传学和生物化学研究。除了在25℃和35℃均能在基本培养基上生长的完全和部分回复突变体外，还获得了能在25℃基本培养基上生长但在35℃不能生长的温度敏感型回复突变体。其中一个回复突变体已被证明在两个温度下均缺乏生物合成脱氢奎尼酸酶活性（在25℃利用可诱导的分解代谢同工酶生长），仅在25℃具有脱氢莽草酸还原酶活性，并且形成了分子量约为野生型一半的芳香族聚合体。这些结果被解释为表明芳香族基因簇中的多效性突变体可能由错义突变导致，也可能如先前研究所表明的由无义突变导致。
