Case M E, Giles N H
Proc Natl Acad Sci U S A. 1971 Jan;68(1):58-62. doi: 10.1073/pnas.68.1.58.
Molecular weights of enzymically active arom aggregates produced by pleiotropic and various arom-1 mutants of Neurospora crassa have been estimated by sucrose density gradient centrifugation. In contrast to most single-gene mutants (which produce intact arom multienzyme aggregates of normal molecular weight- about 230,000), pleiotropic mutants that lack two or more of the five enzyme activities in the arom aggregate produce partial arom aggregates of molecular weights ranging from about 60,000 to about 85,000. In addition, certain arom-1 mutants are pleiotropic in producing arom aggregates of about half the normal molecular weight. Experiments with heterocaryons, as well as with mixtures of extracts from various mutants, have provided evidence concerning the presence or absence of interactions (as detectable molecular hybridization) between different arom aggregates. An evaluation has been made of the relation between the genetic location of particular mutants within the arom gene cluster and the size and enzymic content of the arom aggregates they produce. Interpretations concerning the molecular organization of the arom multienzyme aggregate in N. crassa are presented.
通过蔗糖密度梯度离心法估算了粗糙脉孢菌多效性和各种arom-1突变体产生的具有酶活性的芳香族聚集体的分子量。与大多数单基因突变体（产生分子量正常的完整芳香族多酶聚集体，约230,000）不同，芳香族聚集体中缺乏五种酶活性中的两种或更多种的多效性突变体产生分子量范围约为60,000至约85,000的部分芳香族聚集体。此外，某些arom-1突变体在产生约为正常分子量一半的芳香族聚集体方面具有多效性。用异核体以及不同突变体提取物混合物进行的实验，提供了有关不同芳香族聚集体之间是否存在相互作用（如可检测到的分子杂交）的证据。对特定突变体在芳香族基因簇中的遗传位置与其产生的芳香族聚集体的大小和酶含量之间的关系进行了评估。给出了关于粗糙脉孢菌中芳香族多酶聚集体分子组织的解释。
