用单克隆抗体对单纯疱疹病毒进行鉴定和分型
Identification and typing of herpes simplex viruses with monoclonal antibodies.
作者信息
Balachandran N, Frame B, Chernesky M, Kraiselburd E, Kouri Y, Garcia D, Lavery C, Rawls W E
出版信息
J Clin Microbiol. 1982 Jul;16(1):205-8. doi: 10.1128/jcm.16.1.205-208.1982.
Abstract
Monoclonal antibodies which reacted with type-specific antigens of herpes simplex virus type 2 or with antigens shared by herpes simplex virus types 1 and 2 were used in an indirect immunofluorescence assay to type virus isolates and to detect viral antigens in cells obtained from herpetic lesions. Complete concordance was obtained for 42 isolates typed by endonuclease restriction analysis of viral DNA and by indirect immunofluorescence with monoclonal antibodies. Examination of a limited number of ulcerative lesions revealed that indirect immunofluorescence and virus isolation were comparable in detecting herpes simplex virus. The results indicate that monoclonal antibodies can be used to accurately identify and type isolates of herpes simplex virus.
摘要
用与2型单纯疱疹病毒型特异性抗原或1型和2型单纯疱疹病毒共有的抗原发生反应的单克隆抗体，通过间接免疫荧光试验对病毒分离株进行分型，并检测疱疹病变细胞中的病毒抗原。通过病毒DNA的核酸内切酶限制性分析和单克隆抗体间接免疫荧光对42株分离株进行分型，结果完全一致。对有限数量的溃疡性病变进行检查发现，间接免疫荧光和病毒分离在检测单纯疱疹病毒方面具有可比性。结果表明，单克隆抗体可用于准确鉴定单纯疱疹病毒分离株并进行分型。
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