几种小鼠细胞系中内源性C型病毒体产生的定量分析。
Quantitation of endogenous C-type virion production in several murine cell lines.
作者信息
Luftig R B, McMillan P N, Gudger M
出版信息
J Virol. 1974 Oct;14(4):1017-21. doi: 10.1128/JVI.14.4.1017-1021.1974.
Abstract
Quantitation by enumeration of virion particles, measurement of absorbancy at 260 nm, and densitometry on sodium dodecyl sulfate-polyacrylamide gels has shown that mouse L-M cells yielded 7- to 10-fold less endogenous C-type virions than the parental lines, L or L929. The previously noted stimulation of L-M cell virion production by a concomitant 10% increase in fetal calf serum concentration (D. A. Kindig, R. Karp, and W. H. Kirsten, 1968), was not observed.
摘要
通过对病毒粒子进行计数定量、测量260nm处的吸光度以及对十二烷基硫酸钠-聚丙烯酰胺凝胶进行光密度测定，结果显示，小鼠L-M细胞产生的内源性C型病毒粒子比亲本细胞系L或L929少7至10倍。先前观察到的伴随胎牛血清浓度10%的增加对L-M细胞病毒粒子产生的刺激作用（D. A. Kindig、R. Karp和W. H. Kirsten，1968年）未被观察到。
Zotero 插件