大量劳斯肉瘤病毒的生产与纯化。
Production and purification of large amounts of Rous sarcoma virus.
作者信息
Smith R E, Bernstein E H
出版信息
Appl Microbiol. 1973 Mar;25(3):346-53. doi: 10.1128/am.25.3.346-353.1973.
Abstract
Procedures are described for production and purification of large amounts of Rous sarcoma virus. The virus was produced by Rous sarcoma virus-transformed chicken embryo fibroblasts in roller culture which produced up to 6 mg of virus per day per liter of supernatant fluid. Various methods of concentrating virus were evaluated; pelleting yielded the best results in terms of recovery of infectious virus. Purification was achieved by means of successive velocity and equilibrium density centrifugation by using sucrose solutions made in low-salt buffer. A rapid method for the optical density measurement of virus concentration was also developed.
摘要
本文描述了大量劳斯肉瘤病毒的生产和纯化程序。该病毒由劳斯肉瘤病毒转化的鸡胚成纤维细胞在转瓶培养中产生，每升上清液每天可产生多达6毫克病毒。评估了多种浓缩病毒的方法；就传染性病毒的回收率而言，沉淀法效果最佳。通过在低盐缓冲液中制备的蔗糖溶液，采用连续速度离心和平衡密度离心的方法实现了纯化。还开发了一种快速测量病毒浓度光密度的方法。
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Comparison of immature (rapid harvest) and mature Rous sarcoma virus particles.未成熟（快速收获）的劳斯肉瘤病毒颗粒与成熟劳斯肉瘤病毒颗粒的比较。
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