Lutmer R F, Parsons D, Glueck C J, Morrison J A, Stewart L, Brazier J B, Buncher C R, Ishikawa T T
J Lipid Res. 1974 Nov;15(6):611-4.
A micromethod for quantitation of alpha-lipoprotein cholesterol was devised for gas-liquid chromatography to minimize plasma sample size and facilitate lipid studies using capillary blood from children or small animals. alpha-Lipoprotein cholesterol was measured by gas-liquid chromatography in 20 micro l of the centrifuged supernate obtained after addition of 5 micro l of mixed heparin-manganese chloride solution 1:1 (v/v) to 50 micro l of plasma. Comparison of venous alpha-lipoprotein cholesterol measured by gas-liquid chromatography with venous alpha-lipoprotein cholesterol measured by conventional heparin-manganese precipitation and ferric chloride (colorimetric) cholesterol determination gave a correlation coefficient of 0.98 for 80 plasma samples. Capillary alpha-lipoprotein cholesterol and venous alpha-lipoprotein cholesterol were closely correlated in 31 patients (r = 0.97).
设计了一种用于气液色谱法定量α-脂蛋白胆固醇的微量方法，以尽量减少血浆样本量，并便于使用儿童或小动物的毛细血管血进行脂质研究。在向50微升血浆中加入5微升1:1（v/v）混合肝素-氯化锰溶液后获得的20微升离心上清液中，用气液色谱法测定α-脂蛋白胆固醇。对80份血浆样本进行气液色谱法测定的静脉α-脂蛋白胆固醇与传统肝素-锰沉淀法和氯化铁（比色法）胆固醇测定法测定的静脉α-脂蛋白胆固醇进行比较，相关系数为0.98。31例患者的毛细血管α-脂蛋白胆固醇与静脉α-脂蛋白胆固醇密切相关（r = 0.97）。
