Koliais S I, Dimmock N J
J Virol. 1974 Nov;14(5):1035-9. doi: 10.1128/JVI.14.5.1035-1039.1974.
The kinetics of appearance of an RNA-dependent RNA polymerase activity obtained from human embryo lung cells infected with rhinovirus type 2 have been followed by analysis of the RNA synthesized by the polymerase preparation in vitro. Little single-stranded RNA was synthesized and the proportion of replicative intermediate to replicative form was over threefold greater than obtained in vivo. The polymerase activity in vivo declined in the presence of cycloheximide showing that continued protein synthesis was necessary to maintain RNA replication.
通过分析从感染2型鼻病毒的人胚肺细胞中获得的RNA依赖性RNA聚合酶活性的出现动力学，对该聚合酶制剂在体外合成的RNA进行了跟踪研究。合成的单链RNA很少，复制中间体与复制形式的比例比体内获得的比例高出三倍多。体内的聚合酶活性在环己酰亚胺存在下下降，这表明持续的蛋白质合成对于维持RNA复制是必要的。
