Yin F H, Knight E
J Virol. 1972 Jul;10(1):93-8. doi: 10.1128/JVI.10.1.93-98.1972.
HeLa cells infected with human rhinovirus type 2 synthesize a mixture of single-and double-stranded ribonucleic acid (RNA). The RNA synthesized by the membrane-bound RNA polymerase complex in vitro is also a mixture of single- and double-stranded RNA, whereas the deoxycholate-treated RNA polymerase complex synthesized only double-stranded RNA. Although twice as much cell-associated viral RNA is synthesized in vivo at 34 C than at 37 C, there is no difference in the rate of RNA synthesized in vitro at 34 C and 37 C by the polymerase complex. The RNA polymerase complex, after treatment with deoxycholate, sediments as a broad peak with an average sedimentation value of 120S.
感染人鼻病毒2型的海拉细胞合成单链和双链核糖核酸（RNA）的混合物。膜结合RNA聚合酶复合体在体外合成的RNA也是单链和双链RNA的混合物，而经脱氧胆酸盐处理的RNA聚合酶复合体只合成双链RNA。尽管在体内34℃时合成的细胞相关病毒RNA是37℃时的两倍，但在34℃和37℃下，聚合酶复合体在体外合成RNA的速率没有差异。经脱氧胆酸盐处理后的RNA聚合酶复合体沉降为一个宽峰，平均沉降值为120S。
