禽成髓细胞瘤病毒（AMV）化学修饰的70S RNA对AMV DNA聚合酶活性的影响。
Effect of chemically modified 70S RNA from avian myeloblastosis virus (AMV) upon the activity of AMV DNA polymerase.
作者信息
Papas T S, Chirikjian J G, Pry T W, Massicot J G, Irwin R D, Chirigos M A
出版信息
J Virol. 1974 Nov;14(5):1108-14. doi: 10.1128/JVI.14.5.1108-1114.1974.
Abstract
Adenine residues of 70S avian myeloblastosis virus (AMV) RNA are modified when reacted with chloroacetaldehyde. This modification introduces characteristic fluorescent epsilon-adenosine (epsilonA) probes which were used to monitor the reaction. Under suitable conditions, modified 70S(epsilonA) RNA was maintained intact and was inactive as a template for the AMV DNA polymerase. Furthermore, it inhibited the reaction catalyzed by AMV polymerase when 70S RNA was used as template-primer and had no effect on the two tested bacterial polymerases. Protection against the 70S (epsilonA) RNA inhibition was observed when 70S RNA was primed with oligo(dT) indicating preference of the polymerase for the oligo(dT) primed regions.
摘要
70S禽成髓细胞瘤病毒（AMV）RNA的腺嘌呤残基与氯乙醛反应时会发生修饰。这种修饰引入了特征性荧光ε-腺苷（εA）探针，用于监测反应。在合适的条件下，修饰后的70S（εA）RNA保持完整，并且作为AMV DNA聚合酶的模板无活性。此外，当70S RNA用作模板引物时，它会抑制AMV聚合酶催化的反应，而对两种测试的细菌聚合酶没有影响。当70S RNA用寡聚（dT）引发时，观察到对70S（εA）RNA抑制的保护作用，这表明聚合酶对寡聚（dT）引发区域有偏好。
相似文献
1
Effect of chemically modified 70S RNA from avian myeloblastosis virus (AMV) upon the activity of AMV DNA polymerase.禽成髓细胞瘤病毒（AMV）化学修饰的70S RNA对AMV DNA聚合酶活性的影响。
J Virol. 1974 Nov;14(5):1108-14. doi: 10.1128/JVI.14.5.1108-1114.1974.
2
RNA-dependent DNA polymerase activity of RNA tumor virus. VI. Processive mode of action of avian myeloblastosis virus polymerase.RNA肿瘤病毒的RNA依赖性DNA聚合酶活性。VI. 禽成髓细胞瘤病毒聚合酶的持续作用模式。
J Virol. 1976 Sep;19(3):932-9. doi: 10.1128/JVI.19.3.932-939.1976.
3
Inhibition of AMV DNA polymerase by polyriboadenylic acid containing epsilon-adenosine residues.含ε-腺苷残基的聚核糖腺苷酸对禽成髓细胞瘤病毒DNA聚合酶的抑制作用。
Biochem Biophys Res Commun. 1974 Jul 24;59(2):489-95. doi: 10.1016/s0006-291x(74)80006-9.
4
Comparative ability of RNA and DNA to prime DNA synthesis in vitro: role of sequence, sugar, and structure of template-primer.RNA和DNA在体外引发DNA合成的比较能力：模板引物的序列、糖和结构的作用
Biochemistry. 1975 Apr 8;14(7):1412-25. doi: 10.1021/bi00678a011.
5
Mechanism of interaction of avian myeloblastosis virus reverse transcriptase with avian myeloblastosis virus RNA.禽成髓细胞瘤病毒逆转录酶与禽成髓细胞瘤病毒RNA的相互作用机制
J Virol. 1976 Apr;18(1):26-33. doi: 10.1128/JVI.18.1.26-33.1976.
6
Mechanistic independence of avian myeloblastosis virus DNA polymerase and ribonuclease H.禽成髓细胞瘤病毒DNA聚合酶与核糖核酸酶H的机制独立性
J Virol. 1974 Dec;14(6):1494-502. doi: 10.1128/JVI.14.6.1494-1502.1974.
7
5-Mercaptopolyuridylic acid (MPU), a potent inhibitor of the reverse transcriptase from avian myeloblastosis virus.5-巯基聚尿苷酸（MPU），一种来自禽成髓细胞瘤病毒的逆转录酶的强效抑制剂。
Res Commun Chem Pathol Pharmacol. 1982 May;36(2):215-28.
8
Stepwise dissociation of high molecular weight avian myeloblastosis virus RNA: 30-40S RNA subunits--the best natural template-primer for viral reverse transcriptase.高分子量禽成髓细胞瘤病毒RNA的逐步解离：30 - 40S RNA亚基——病毒逆转录酶的最佳天然模板引物。
J Gen Virol. 1976 Feb;30(2):187-95. doi: 10.1099/0022-1317-30-2-187.
9
Avian myeloblastosis virus DNA polymerase: initiation of DNA synthesis and an associated ribonuclease.禽成髓细胞瘤病毒DNA聚合酶：DNA合成的起始及一种相关的核糖核酸酶
Johns Hopkins Med J Suppl. 1973;2:193-8.
10
RNA-DNA covalent bonds between the RNA primers and the DNA products formed by RNA tumor virus DNA polymerase.RNA肿瘤病毒DNA聚合酶形成的RNA引物与DNA产物之间的RNA-DNA共价键。
J Virol. 1973 Dec;12(6):1491-502. doi: 10.1128/JVI.12.6.1491-1502.1973.
引用本文的文献
1
Affinity chromatography of viral DNA polymerases on pyran-sepharose.病毒DNA聚合酶在吡喃琼脂糖上的亲和层析
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1142-6. doi: 10.1073/pnas.72.3.1142.
2
New observations concerning the chloroacetaldehyde reaction with some tRNA constituents. Stable intermediates, kinetics and selectivity of the reaction.关于氯乙醛与某些tRNA成分反应的新观察结果。反应的稳定中间体、动力学和选择性。
Nucleic Acids Res. 1978 Mar;5(3):789-804. doi: 10.1093/nar/5.3.789.
本文引用的文献
1
Inhibition of the DNA polymerase of Rauscher leukemia virus by single-stranded polyribonucleotides.单链多聚核糖核苷酸对劳氏肉瘤病毒DNA聚合酶的抑制作用
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2198-202. doi: 10.1073/pnas.68.9.2198.
2
Synthesis and properties of a new fluorescent polynucleotide, poly(1,N6-ethenoadenylic acid).一种新型荧光多核苷酸聚（1，N6-乙烯腺苷酸）的合成与性质
Biochim Biophys Acta. 1973 Apr 21;308(7):28-34. doi: 10.1016/0005-2787(73)90118-4.
3
Fluorescent adenosine and cytidine derivatives.荧光腺苷和胞苷衍生物
Biochem Biophys Res Commun. 1972 Jan 31;46(2):597-604. doi: 10.1016/s0006-291x(72)80181-5.
4
Evidence that the polyadenylic acid segment of "35S" RNA of avian myeloblastosis virus is located at the 3'-OH terminus.禽成髓细胞瘤病毒“35S”RNA的聚腺苷酸片段位于3'-OH末端的证据。
Biochem Biophys Res Commun. 1973 Nov 1;55(1):8-16. doi: 10.1016/s0006-291x(73)80052-x.
5
RNA-directed DNA polymerase of Rous sarcoma virus: initiation of synthesis with 70 S viral RNA as template.劳氏肉瘤病毒的RNA指导DNA聚合酶：以70S病毒RNA为模板起始合成
J Mol Biol. 1973 Sep 5;79(1):163-83. doi: 10.1016/0022-2836(73)90277-5.
6
The 3'-terminal nucleosides of the high molecular weight RNA of avian myeloblastosis virus.禽成髓细胞瘤病毒高分子量RNA的3'末端核苷。
Proc Natl Acad Sci U S A. 1972 May;69(5):1176-80. doi: 10.1073/pnas.69.5.1176.
7
Purification of the DNA polymerase of avian myeloblastosis virus.禽成髓细胞瘤病毒DNA聚合酶的纯化
Biochim Biophys Acta. 1971 Sep 24;246(3):365-83. doi: 10.1016/0005-2787(71)90773-8.
8
RNA-dependent DNA polymerase in viruses and cells: views on the current state.病毒和细胞中的RNA依赖性DNA聚合酶：当前状态的观点
Blood. 1972 Jan;39(1):117-37.
9
Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases.区分RNA肿瘤病毒的逆转录酶与其他已知的DNA聚合酶。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2203-6. doi: 10.1073/pnas.68.9.2203.
10
Viral DNA-dependent DNA polymerase and the properties of thymidine labelled material in virions of an oncogenic RNA virus.致癌RNA病毒粒子中的病毒DNA依赖性DNA聚合酶及胸腺嘧啶标记物质的特性
Nature. 1970 Oct 31;228(5270):427-30. doi: 10.1038/228427a0.
Zotero 插件