猴病毒40的转录。II. 从不同转化细胞系中提取的RNA与猴病毒40 DNA单链的杂交。
Transcription of simian virus 40. II. Hybridization of RNA extracted from different lines of transformed cells to the separated strands of simian virus 40 DNA.
作者信息
Ozanne B, Sharp P A, Sambrook J
出版信息
J Virol. 1973 Jul;12(1):90-8. doi: 10.1128/JVI.12.1.90-98.1973.
Abstract
The amount of simian virus 40 (SV40) DNA present in various SV40-transformed mouse cell lines and "revertants" isolated from them was determined. The number of viral DNA copies in the different cell lines ranged from 1.35 to 8.75 copies per diploid quantity of mouse cell DNA and from 2.2 to 14 copies per cell. The revertants had the same number of viral DNA copies per diploid quantity of mouse cell DNA as their parental cell lines. (However, they showed an increased number of viral DNA copies per cell due to their increased amount of DNA.) By using separated strands of SV40 DNA, the extent of each DNA strand transcribed into stable RNA species was determined for the transformed and "revertant" cell lines. From 30 to 80% of the "early" strand and from 0 to 20% of the "late" strand was present as stable RNA species in the cell lines tested. There was no alteration in the pattern of the stable viral RNA species present in three concanavalin A-selected revertants, whereas in a fluorodeoxyuridine-selected revertant there appeared to be less viral-specific RNA present in the cells.
摘要
测定了存在于各种经猿猴病毒40（SV40）转化的小鼠细胞系以及从这些细胞系中分离出的“回复突变体”中的SV40 DNA量。不同细胞系中病毒DNA拷贝数范围为每二倍体小鼠细胞DNA 1.35至8.75个拷贝，以及每个细胞2.2至14个拷贝。回复突变体每二倍体小鼠细胞DNA中的病毒DNA拷贝数与其亲代细胞系相同。（然而，由于其DNA量增加，它们每个细胞中的病毒DNA拷贝数有所增加。）通过使用SV40 DNA的单链，测定了转化细胞系和“回复突变体”细胞系中每条DNA链转录为稳定RNA种类的程度。在所测试的细胞系中，30%至80%的“早期”链和0%至20%的“晚期”链以稳定RNA种类存在。在三种经刀豆球蛋白A选择的回复突变体中，存在的稳定病毒RNA种类模式没有改变，而在一种经氟脱氧尿苷选择的回复突变体中，细胞中似乎存在较少的病毒特异性RNA。
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