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从用c-H-ras癌基因转化的NIH 3T3细胞中分离隐性（中介体）回复突变体。

Isolation of recessive (mediator-) revertants from NIH 3T3 cells transformed with a c-H-ras oncogene.

作者信息

Yamada H, Omata-Yamada T, Wakabayashi-Ito N, Carter S G, Lengyel P

机构信息

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut 06511.

出版信息

Mol Cell Biol. 1990 Apr;10(4):1822-7. doi: 10.1128/mcb.10.4.1822-1827.1990.

DOI:10.1128/mcb.10.4.1822-1827.1990
PMID:2181289
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC362294/
Abstract

We have generated two serum- and anchorage-dependent revertants from NIH 3T3 cells transformed with multiple copies of the human c-H-ras oncogene. In both revertants, the c-H-ras oncogene was fully expressed. Fusion of either revertant with untransformed cells or of the two revertants with one another resulted in transformed progeny. These results indicated that the two revertants were recessive and in different complementation groups. We believe that in our two revertants some of the genes mediating the transforming activity of the c-H-ras oncogene are defective; we are attempting to identify these mediator genes.

摘要

我们从用多拷贝人c-H-ras癌基因转化的NIH 3T3细胞中获得了两种血清和锚定依赖型回复突变体。在这两种回复突变体中，c-H-ras癌基因均充分表达。任一回复突变体与未转化细胞融合，或两个回复突变体相互融合，均产生转化后代。这些结果表明这两种回复突变体是隐性的，且属于不同的互补群。我们认为在我们的这两种回复突变体中，一些介导c-H-ras癌基因转化活性的基因存在缺陷；我们正试图鉴定这些介导基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a0/362294/6d69ae5752b1/molcellb00040-0536-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a0/362294/c3647817f9a5/molcellb00040-0533-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a0/362294/c7b386b6ee90/molcellb00040-0534-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a0/362294/f58a0921d663/molcellb00040-0535-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a0/362294/6d69ae5752b1/molcellb00040-0536-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a0/362294/c3647817f9a5/molcellb00040-0533-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a0/362294/c7b386b6ee90/molcellb00040-0534-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a0/362294/f58a0921d663/molcellb00040-0535-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b6a0/362294/6d69ae5752b1/molcellb00040-0536-a.jpg

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Isolation of recessive (mediator-) revertants from NIH 3T3 cells transformed with a c-H-ras oncogene.从用c-H-ras癌基因转化的NIH 3T3细胞中分离隐性（中介体）回复突变体。
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本文引用的文献

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Flat revertants isolated from Kirsten sarcoma virus-transformed cells are resistant to the action of specific oncogenes.从 Kirsten 肉瘤病毒转化细胞中分离出的扁平回复突变体对特定癌基因的作用具有抗性。
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基因生态学：由于染色体中基因的空间排列而导致的顺式作用基因与基因之间的相互作用影响相邻转染的c-H-ras表达。
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Novel revertants of H-ras oncogene-transformed R6-PKC3 cells.H-ras癌基因转化的R6-PKC3细胞的新型回复突变体。
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Plasmid-encoded hygromycin B resistance: the sequence of hygromycin B phosphotransferase gene and its expression in Escherichia coli and Saccharomyces cerevisiae.质粒编码的潮霉素B抗性：潮霉素B磷酸转移酶基因的序列及其在大肠杆菌和酿酒酵母中的表达。
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"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.“蛋白质免疫印迹法”：蛋白质从十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳转移至未修饰的硝酸纤维素膜上，并用抗体和放射性碘化蛋白A进行放射自显影检测。
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Methods for obtaining revertants of transformed cells.获得转化细胞回复突变体的方法。
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