Tschider S R, Berryhill D L, Schipper I A
Appl Microbiol. 1974 Dec;28(6):1030-2. doi: 10.1128/am.28.6.1030-1032.1974.
A membrane adsorption procedure was used to concentrate infectious bovine rhinotracheitis virus from 1-liter quantities of distilled water. Cellulose nitrate membrane filters (0.45-mum pore size) efficiently adsorbed this herpesvirus from water, and virus was recovered from the membrane by elution with 10 ml of fetal calf serum during sonic treatment. The average recovery rate was 70%.
采用膜吸附法从1升蒸馏水中浓缩感染性牛鼻气管炎病毒。硝酸纤维素膜过滤器(孔径0.45微米)能有效地从水中吸附这种疱疹病毒,在超声处理过程中用10毫升胎牛血清洗脱可从膜上回收病毒。平均回收率为70%。