肠道病毒在膜滤器上的富集
Concentration of enteroviruses on membrane filters.
作者信息
Wallis C, Melnick J L
出版信息
J Virol. 1967 Jun;1(3):472-7. doi: 10.1128/JVI.1.3.472-477.1967.
Abstract
Enteroviruses can be made to adsorb or to pass through membrane filters by manipulation of the suspending medium. Salts facilitate virus adsorption, but membrane-coating components (MCC) interfere. Because cells release MCC into the culture medium during viral growth, MCC must be removed before virus can be adsorbed to membranes. Adsorbed virus can be eluted with diluents containing MCC (cell extracts or serum) or agents that reduce surface tension (sodium lauryl sulfate). By membrane adsorption and elution, enteroviruses can be readily concentrated and quantitatively recovered from crude virus harvests.
摘要
通过对悬浮介质进行处理，可使肠道病毒吸附或通过膜滤器。盐类有助于病毒吸附，但膜包被成分（MCC）会产生干扰。由于细胞在病毒生长过程中会将MCC释放到培养基中，因此在病毒吸附到膜上之前必须去除MCC。吸附的病毒可用含有MCC的稀释剂（细胞提取物或血清）或降低表面张力的试剂（十二烷基硫酸钠）洗脱。通过膜吸附和洗脱，可轻松从粗病毒收获物中浓缩并定量回收肠道病毒。
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