实验性犬腺病毒肾小球肾炎中免疫复合物的系膜定位。

Wright N G, Morrison W I, Thompson H, Cornwell H J

Br J Exp Pathol. 1974 Oct;55(5):458-65.

Each of a group of 14 dogs was infected experimentally by an intravenous dose of canine adenovirus calculated to allow survival until the initial stages of antibody production; the kidneys of infected dogs were examined during the period of 4-14 days after administration of virus. Proliferative glomerulonephritis with localization of IgG, C3 and viral antigen in mesangial regions was demonstrated. With the electron microscope, electron dense deposits were found scattered throughout the mesangium. There was proliferation of mesangial cells, infiltration into the glomerular tuft of polymorphonuclear leucocytes and, in some cases, focal glomerular necrosis with intracapsular and tubular haemorrhage. By means of an indirect immunofluorescence test, anti-viral antibody was detected in kidney eluates; anti-kidney antibody was not present.

一组14只狗，每只通过静脉注射一定剂量的犬腺病毒进行实验性感染，该剂量经计算可使狗存活至抗体产生的初始阶段；在接种病毒后的4至14天内，对受感染狗的肾脏进行检查。结果显示，肾小球系膜区出现IgG、C3和病毒抗原定位的增殖性肾小球肾炎。通过电子显微镜观察，发现电子致密沉积物散在于整个系膜中。系膜细胞增生，多形核白细胞浸润到肾小球丛，在某些情况下，还出现局灶性肾小球坏死并伴有囊内和肾小管出血。通过间接免疫荧光试验，在肾脏洗脱液中检测到抗病毒抗体；未检测到抗肾抗体。