Holden R J, Ferguson A
Gut. 1979 Mar;20(3):234-9. doi: 10.1136/gut.20.3.234.
A technique of microdissection of colonic mucosa has allowed the study of mitotic activity, measured by metaphase accumulation following colchicine blockade, in individual crypts of mouse colon. The changes occurring during growth and development of normal colon have been studied and compared with changes found in antigen free colon (colonic isografts) and in cell-mediated immune damage of the bowel (allograft rejection). Metaphase accumulation was steady at two metaphases per hour in baby mouse colon until 18 days after birth. Between 18 and 24 days a rapid, and significant increase in mitotic activity occurred (P less than 0.01), reached adult values, and changed no further. Metaphase accumulation in isografts was similar to normal colon for the first two weeks after transplantation but the rise in mitotic activity in the third week did not occur. Allografts of colon showed two- to three-fold increases in metaphase accumulation when compared with both normal colon and isografts (P less than 0.01). When crypt mitotic activity was compared with the length of crypts measured in histological sections of normal colon, isografts, and allografts, no clear relationship was observed. Both changes in the luminal environment of the gut at the time of weaning and cell-mediated immune reactions in the colonic wall appeared to be associated with increased mitotic activity in colonic crypts.
一种结肠黏膜显微切割技术能够研究小鼠结肠单个隐窝中的有丝分裂活性，该活性通过秋水仙碱阻断后中期积累来测定。已对正常结肠生长和发育过程中发生的变化进行了研究，并与无抗原结肠（结肠同基因移植）以及肠道细胞介导免疫损伤（同种异体移植排斥）中发现的变化进行了比较。在出生后18天之前，幼鼠结肠中的中期积累稳定在每小时两个中期。在18至24天之间，有丝分裂活性迅速且显著增加（P小于0.01），达到成年值，且不再进一步变化。同基因移植在移植后的前两周内中期积累与正常结肠相似，但在第三周没有出现有丝分裂活性的升高。与正常结肠和同基因移植相比，结肠同种异体移植的中期积累增加了两到三倍（P小于0.01）。当将隐窝有丝分裂活性与在正常结肠、同基因移植和同种异体移植的组织学切片中测量的隐窝长度进行比较时，未观察到明显的关系。断奶时肠道腔内环境的变化以及结肠壁中的细胞介导免疫反应似乎都与结肠隐窝中有丝分裂活性的增加有关。
