Sanderson C J, Franks D
Immunology. 1974 Dec;27(6):1045-51.
Fourteen days after injection with Detroit-6 cells (a human cell line) rats have high titres of antibody capable of inducing cytotoxicity towards Detroit-6 cells in normal spleen cells. At the same time, the spleen cells from the injected rats are cytotoxic for Detroit-6 cells . However, killing by allergized spleen cells is apparently not entirely antibody induced, since heterologous anti-thymocyte sera (absorbed with rat bone marrow cells) inhibit target cell killing by spleen cells from injected rats but do not appreciably inhibit killing of antibody-coated target cells by normal spleen cells. Wistar spleen cells `activated' by transfer into irradiated August rats are cytotoxic for August hepatoma cells. This cytotoxicity is also inhibited by the absorbed anti-thymocyte sera. It is suggested that the cytotoxicity of spleen cells , in both these allogeneic and xenogeneic systems, is mediated by thymus-derived (T) cells.
注射底特律 - 6细胞(一种人类细胞系)14天后,大鼠体内产生了高滴度的抗体,这种抗体能够在正常脾细胞中诱导对底特律 - 6细胞的细胞毒性。同时,注射大鼠的脾细胞对底特律 - 6细胞具有细胞毒性。然而,致敏脾细胞的杀伤作用显然并非完全由抗体诱导,因为异源抗胸腺细胞血清(用大鼠骨髓细胞吸收)可抑制注射大鼠脾细胞对靶细胞的杀伤,但对正常脾细胞杀伤抗体包被的靶细胞的作用没有明显抑制。通过转移到经辐照的奥古斯塔大鼠体内而“激活”的Wistar脾细胞对奥古斯塔肝癌细胞具有细胞毒性。这种细胞毒性也被吸收的抗胸腺细胞血清所抑制。有人提出,在这两种同种异体和异种系统中,脾细胞的细胞毒性是由胸腺来源的(T)细胞介导的。