Sanderson C J, Taylor G A
Immunology. 1976 Jan;30(1):117-21.
Experiments are described in which the inactivation of macrophage activity by quartz particles is used to investigate the role of macrophages in antibody-dependent cell-mediated cytotoxicity in the rat. The results confirm previous findings which indicated that macrophages play no significant role in assays using cell line cells as targets. On the other hand, previous suggestions that macrophages are active against antibody-coated chick erythrocytes cannot be substantiated. In fact, it is shown that macrophages can play a protective role, and that inhibiting macrophage activity in peritoneal exudates leads to a spectacular increase in antibody-dependent lysis of chick erythrocytes. It is suggested that the confusion surrounding the role of macrophages in this assay has resulted from the failure to recognize that adherence techniques such as carbonyl-iron treatment of cell suspensions can result in substantial depletion of non-phagocytic adherent cells.
本文描述了一些实验，其中利用石英颗粒使巨噬细胞活性失活，以研究巨噬细胞在大鼠抗体依赖性细胞介导的细胞毒性中的作用。结果证实了先前的发现，即巨噬细胞在以细胞系细胞为靶标的检测中不起重要作用。另一方面，先前认为巨噬细胞对抗体包被的鸡红细胞有活性的观点无法得到证实。事实上，研究表明巨噬细胞可以起到保护作用，并且抑制腹膜渗出液中的巨噬细胞活性会导致鸡红细胞抗体依赖性裂解显著增加。有人认为，围绕巨噬细胞在该检测中的作用的混淆源于未能认识到诸如用羰基铁处理细胞悬液等黏附技术会导致非吞噬性黏附细胞大量减少。
