Kozik M B, Gramza G, Sikorska Z
Acta Histochem. 1979;64(1):55-63. doi: 10.1016/S0065-1281(79)80052-5.
Experiments were performed aiming at establishing optimal conditions for demonstrating the activity of dehydrogenases in Epon-embedded, semithin sections. Small fragments of the nervous tissue were incubated 1, 4, 10 and 24 h 37, 20 and 4 degrees C in the presence of NBT or TC NBT. The incubated fragments were embedded in Epon and semithin sections prepared thereof. The activities of succinate dehydrogenase and of alpha-glycerol dehydrogenase was evaluated. The experiments have shown that semithin, Epon-embedded sections may be used for demonstration and cytological localization of respiratory enzymes. Various experimental conditions were tried. Optimal results obtained when unfixed tissue fragments were incubated for 24 h at 4 degrees C.
进行了旨在确定在环氧树脂包埋的半薄切片中显示脱氢酶活性的最佳条件的实验。将神经组织的小碎片在37℃、20℃和4℃下，于存在NBT或TC NBT的情况下孵育1、4、10和24小时。将孵育后的碎片包埋在环氧树脂中并制备半薄切片。评估琥珀酸脱氢酶和α-甘油脱氢酶的活性。实验表明，环氧树脂包埋的半薄切片可用于呼吸酶的显示和细胞定位。尝试了各种实验条件。当未固定的组织碎片在4℃下孵育24小时时获得了最佳结果。
