抗核苷抗体的特异性
Specificity of anti-nucleoside antibodies.
作者信息
Humayun M Z, Jacob T M
出版信息
Biochem J. 1974 Jul;141(1):313-5. doi: 10.1042/bj1410313.
Abstract
The method of conjugation of a nucleoside or related compound to a carrier protein may have a significant effect on the specificity of the antibodies elicited. It is demonstrated, by means of the membrane-filtration assay, that anti-isopentenyladenosine antibodies produced by the ;periodate procedure' are much more reactive with the periodate-oxidized form of the nucleoside than with the parent compound. In addition, the simplicity and specificity of the assay used suggests its use as a sensitive radioimmunoassay for this multifunctional nucleoside.
摘要
将核苷或相关化合物与载体蛋白偶联的方法可能会对所引发抗体的特异性产生显著影响。通过膜过滤分析表明，“高碘酸盐法”产生的抗异戊烯基腺苷抗体与核苷的高碘酸盐氧化形式的反应性比与母体化合物的反应性高得多。此外，所用分析方法的简单性和特异性表明它可作为这种多功能核苷的灵敏放射免疫分析方法。
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