Drocourt J L, Leng M
Eur J Biochem. 1975 Aug 1;56(1):149-55. doi: 10.1111/j.1432-1033.1975.tb02217.x.
Antibodies to adenosine-5'-monophosphate were produced in rabbits by injecting a conjugate of the nucleotide (oxidized with periodate) with bovine serum albumin. Nucleotide-specific antibodies were isolated by affinity chromatography on oligoadenylic acids/agarose column. Pure immunoglobulin G antibodies were obtained by gel filtration on Sephadex G-200. These antibodies, as analyzed by double diffusion react with adenosine 5'-monophosphate--bovine serum albumin, slightly with inosine-5'-monophosphate conjugate and not at all with the other nucleotide conjugates. The association constants for adenosine-5'-monophosphate--antibody complex formation obtained by dialysis equilibrium and fluorescence measurements, are in good agreement. This latter technique was used to study on one hand the influence of temperture and salt on complex formation, on the other hand the interaction of the antibodies with AMP derivatives. The phosphate group, the ribose and the base are recognized by the antibody, but the C-8 atom of adenine residues is not directly involved in the binding.
通过向兔体内注射经高碘酸盐氧化的核苷酸与牛血清白蛋白的偶联物，制备出了抗5'-单磷酸腺苷的抗体。核苷酸特异性抗体通过在寡聚腺苷酸/琼脂糖柱上进行亲和层析分离得到。通过在葡聚糖凝胶G - 200上进行凝胶过滤获得了纯免疫球蛋白G抗体。经双向扩散分析，这些抗体与5'-单磷酸腺苷 - 牛血清白蛋白反应，与5'-单磷酸次黄嘌呤偶联物有轻微反应，与其他核苷酸偶联物则完全不反应。通过透析平衡和荧光测量得到的5'-单磷酸腺苷 - 抗体复合物形成的缔合常数吻合良好。后一种技术一方面用于研究温度和盐对复合物形成的影响，另一方面用于研究抗体与AMP衍生物的相互作用。抗体能识别磷酸基团、核糖和碱基，但腺嘌呤残基的C - 8原子不直接参与结合。
