Ali M A, Jacob T M
Immunol Invest. 1985 Feb;14(1):85-94. doi: 10.3109/08820138509052448.
Antibodies to adenosine were elicited in rabbits using a conjugate of adenosine (periodate oxidized) and bovine serum albumin as immunogen. The conjugate was found to be of a higher molecular size than the original protein, apparently due to the cross-linking of albumin molecules by the dialdehydes generated during the periodate oxidation of adenosine. It produced high titres of antibodies, which were 400-fold more reactive to adenosine-trialcohol than to adenosine, when measured by a nitrocellulose filter assay. In addition, a specific elution procedure for the isolation of hapten-specific antibodies is described.
以高碘酸盐氧化的腺苷与牛血清白蛋白的偶联物作为免疫原，在兔体内诱导产生抗腺苷抗体。发现该偶联物的分子大小比原始蛋白质更大，这显然是由于腺苷高碘酸盐氧化过程中产生的二醛使白蛋白分子发生了交联。它产生了高滴度的抗体，通过硝酸纤维素滤膜测定法测量，这些抗体对腺苷三醇的反应性比对腺苷的反应性高400倍。此外，还描述了一种用于分离半抗原特异性抗体的特定洗脱程序。
