Saddler J N, Khan A W
Can J Microbiol. 1979 Dec;25(12):1427-32. doi: 10.1139/m79-222.
A mesophilic anaerobe, a member of the Bacteroidaceae family (NRC2248), isolated from a cellulose-enrichment culture, digested untreated Whatman cellulose powder and HCl-treated cotton battings while producing hydrogen, carbon dioxide, cellobiose, glucose, and acetic acid as the sole volatile acid. This organism also utilized cellobiose as carbon and energy source but did not utilize glucose. It grew well in synthetic medium containing ammonium salts as nitrogen source and having a pH value of 7.0-7.1 and an Eh value of -160mV or lower. The nutrient requirements of this organism were found to be similar to those of other anaerobes except for Na2S which inhibited cellulose degradation in concentrations above 0.75 mM. Best cellulose degradation occurred under an atmosphere of 80% N2-20% CO2. Use of H2 or 80% H2-20% CO2 as headspace gas inhibited growth. Although accumulation of acetic acid in about 15-16 mM concentrations inhibited the further formation of H2, CO2, and acetic acid in the broth, it did not stop the degradation of cellulose. The results indicate that this organism has the ability to grow in media containing up to 20 g/L of cellulose and to produce industrially important and easily separable end products from cellulose.
一种嗜温厌氧菌，属于拟杆菌科（NRC2248），从纤维素富集培养物中分离得到，能消化未处理的Whatman纤维素粉末和经盐酸处理的棉絮，同时产生氢气、二氧化碳、纤维二糖、葡萄糖和作为唯一挥发性酸的乙酸。这种微生物还利用纤维二糖作为碳源和能源，但不利用葡萄糖。它在以铵盐作为氮源、pH值为7.0 - 7.1且Eh值为-160mV或更低的合成培养基中生长良好。发现这种微生物的营养需求与其他厌氧菌相似，除了Na2S，其浓度高于0.75 mM时会抑制纤维素降解。在80% N2 - 20% CO2的气氛下纤维素降解效果最佳。使用氢气或80% H2 - 20% CO2作为顶空气体抑制生长。虽然约15 - 16 mM浓度的乙酸积累会抑制肉汤中氢气、二氧化碳和乙酸的进一步形成，但并未阻止纤维素的降解。结果表明，这种微生物有能力在含有高达20 g/L纤维素的培养基中生长，并从纤维素中产生具有工业重要性且易于分离的终产物。
