Khan A W
Can J Microbiol. 1977 Dec;23(12):1700-5. doi: 10.1139/m77-245.
A mixed culture in which cellulose is capable of being converted to methane and carbon dioxide was obtained from an inoculum procured from a sewage-treatment plant and maintained in a synthetic medium containing tissue paper and an inorganic salt and vitamin mixture. The culture was tested for its ability to degrade 12 different paper and cotton products under batch conditions in 3-l anaerobic fermenters. This culture degraded 6-8 mmol/l per week of cellulose, expressed as glucose equivalents, with total gas yields of 0.3 m3/kg of cellulose degraded. The gas produced contained between 56 and 59% of methane. Maximum cellulose degradation occurred at chemical oxygen demand:nitrogen:phosphorus level of 80:5:1 and was adversely affected by high stirring rate. Also the presence of higher proportions of lignin in cellulose products adversely affected the ability of this culture to degrade cellulose.
从污水处理厂获取接种物，并将其保存在含有薄页纸以及无机盐和维生素混合物的合成培养基中，从而得到了一种能将纤维素转化为甲烷和二氧化碳的混合培养物。在3升厌氧发酵罐中，在分批培养条件下测试了该培养物降解12种不同纸张和棉制品的能力。这种培养物每周降解6 - 8毫摩尔/升的纤维素（以葡萄糖当量表示），每降解1千克纤维素的总气体产量为0.3立方米。产生的气体中甲烷含量在56%至59%之间。在化学需氧量:氮:磷为80:5:1的水平下，纤维素降解量最大，且高搅拌速率会对其产生不利影响。此外，纤维素产品中较高比例木质素的存在也会对该培养物降解纤维素的能力产生不利影响。
