硫胺素代谢在中枢神经系统中的作用。II. 各种药剂对大鼠脑硫胺素三磷酸酶活性的影响。

Role of thiamine metabolism in the central nervous system. II. Effects of various agents on thiamine triphosphatase activity in rat brain.

作者信息

Iwata H, Baba A, Matsuda T, Terashita Z, Ishii K

出版信息

Jpn J Pharmacol. 1974 Dec;24(6):825-9. doi: 10.1254/jjp.24.825.

DOI:10.1254/jjp.24.825

Abstract

The effects of various agents on the activity of brain thiamine triphosphatase (TTPase) in vivo and in vitro were studied. Thiamine deficiency caused a significant increase in soluble TTPase activity and a decrease in membrane-associated TTPase activity. Insulin and a fasting state did not affect these enzyme activities. DL-Methamphetamine (10mg/kg i.p.) increased the activity of the soluble TTPase, whereas reserpine (2.5 mg/kg i.p.) caused no change in the enzyme activities. A single injection of chlopromazine (25 mg/kg s.c.) had no effect on the microsomal or soluble TTPase activities, but repeated injections reduced the activity of the microsomal enzyme. The effects of various neuroactive agents on microsomal TTPase activity were examined in vitro. Among the drugs tested, only chlorpromazine caused marked inhibition of the enzyme activity.

摘要

研究了各种药物对体内和体外脑硫胺三磷酸酶（TTPase）活性的影响。硫胺缺乏导致可溶性TTPase活性显著增加，膜相关TTPase活性降低。胰岛素和禁食状态不影响这些酶活性。DL-甲基苯丙胺（10mg/kg腹腔注射）增加了可溶性TTPase的活性，而利血平（2.5mg/kg腹腔注射）对酶活性无影响。单次注射氯丙嗪（25mg/kg皮下注射）对微粒体或可溶性TTPase活性无影响，但重复注射会降低微粒体酶的活性。在体外研究了各种神经活性药物对微粒体TTPase活性的影响。在所测试的药物中，只有氯丙嗪对酶活性有明显抑制作用。