Dahnke H G, Koglin K, Mosebach K O
Hoppe Seylers Z Physiol Chem. 1979 Apr;360(4):543-57.
In the prostate of adult Wistar rats the RNA/DNA quotient of the whole organ as well as the amount of RNA and DNA in the nucleus was measured at different times after castration. Furthermore the half-life time for the turnover of the RNA in the nucleus and the cytoplasm was determined for normal and castrated rats with the aid of pulse labelling using [5(-3)H]uridine. A mathematical model was developed to analyze the experimental results. This model enabled us to make differentiated statements on the heterogeneous nuclear RNA (hmRNA) and the remaining RNA in the nucleus. The evaluation of the experimental values gave the following results: 1. By deprivation of androgens the uptake of [3H]uridine into the prostate is lowered. 2. The amount of DNA in the morphologically intact nucleus remains constant at least up to the 12th day after castration. 3. 6 days after castration the amount of hmRNA decreases to 1/10 and that of cytoplasmic RNA to 1/4. 4. The half-life time for the decrease of the whole nuclear RNA is 3.7 d and that of the cytoplasmic RNA 1.7 d. 5. The half-life time for the turnover of hmRNA is 16 min and that of cytoplasmic RNA about 2 days. 6 days after castration the half-life times are unchanged. The experimental results suggest that the observed decrease of nuclear RNA following castration can mainly be attributed to a reduced synthesis of hnRNA, while the decrease of cytoplasmic RNA is first of all caused by an increase in RNA degradation.
在成年Wistar大鼠的前列腺中，于去势后的不同时间测量了整个器官的RNA/DNA商以及细胞核中RNA和DNA的含量。此外，借助[5(-3)H]尿苷脉冲标记法，测定了正常和去势大鼠细胞核及细胞质中RNA周转的半衰期。建立了一个数学模型来分析实验结果。该模型使我们能够对核不均一RNA（hnRNA）和细胞核中其余RNA做出有区别的阐述。对实验值的评估得出以下结果：1. 通过去除雄激素，[3H]尿苷进入前列腺的摄取量降低。2. 形态完整的细胞核中的DNA量至少在去势后第12天保持恒定。3. 去势6天后，hnRNA的量减少到1/10，细胞质RNA的量减少到1/4。4. 整个细胞核RNA减少的半衰期为3.7天，细胞质RNA的半衰期为1.7天。5. hnRNA周转的半衰期为16分钟，细胞质RNA的半衰期约为2天。去势6天后，半衰期不变。实验结果表明，去势后观察到的细胞核RNA减少主要可归因于hnRNA合成减少，而细胞质RNA的减少首先是由RNA降解增加引起的。
