Zaboĭkin M M, Likhtenshteĭn A V, Shapot V S
Mol Biol (Mosk). 1981 Mar-Apr;15(2):274-80.
It is usually necessary to compare the kinetics of labelling of nucleoside triphosphates and nuclear RNAs to determine the turnover rate (half-life, T1/2) of nuclear RNAs. It is shown that the widely adopted correction for non-constant specific radioactivity of precursor pool is not correct in general and could be used only for very stable RNAs. The method for T1/2 determination is described which is suitable for any form of UTP labelling kinetics. Besides, the criterion was found for revelation of metabolic heterogeneity of nuclear RNA population. Rat liver nuclear DNA-like RNA appeared to be heterogeneous and consisted of two subpopulations, one rapidly labelled with T1/2 about 30 min and other, three times larger, with no labelling during the experiment.
通常有必要比较核苷三磷酸和核RNA的标记动力学，以确定核RNA的周转率（半衰期，T1/2）。结果表明，广泛采用的对前体库非恒定比放射性的校正通常是不正确的，仅适用于非常稳定的RNA。描述了适用于任何形式UTP标记动力学的T1/2测定方法。此外，还发现了揭示核RNA群体代谢异质性的标准。大鼠肝核DNA样RNA似乎是异质的，由两个亚群组成，一个亚群快速标记，T1/2约为30分钟，另一个亚群大3倍，在实验过程中未被标记。
