淋巴细胞中的磷脂酰肌醇裂解。低浓度钙离子的需求及其他阳离子的作用。
Phosphatidylinositol cleavage in lymphocytes. Requirement for calcium ions at a low concentration and effects of other cations.
作者信息
Allan D, Michell R H
出版信息
Biochem J. 1974 Sep;142(3):599-604. doi: 10.1042/bj1420599.
Abstract
The soluble activity in lymphocytes which converts phosphatidylinositol into 1,2-diacylglycerol and inositol phosphates requires Ca(2+) ions. At pH7 maximum activity occurs at Ca(2+) approximately 0.7mum whereas at pH5.5 the equivalent value is approx. 50mum. At Ca(2+) approximately 1mum, a concentration similar to common intracellular values, essentially all activity is confined to the peak of activity at pH7.0. Previous reports of requirements for larger amounts of Ca(2+) may reflect the fact that the Ca(2+)-buffering capacity of phosphatidylinositol means that high substrate concentrations can effectively decrease Ca(2+). Cations which displace Ca(2+) from association with phosphatidylinositol can, at low Ca(2+), enhance enzyme activity. Phosphatidylinositol breakdown in intact cells might be controlled, at least in part, by changes in intracellular Ca(2+).
摘要
淋巴细胞中可将磷脂酰肌醇转化为1,2 - 二酰甘油和肌醇磷酸的可溶性活性需要钙离子。在pH7时，最大活性出现在游离钙离子浓度约为0.7μM时，而在pH5.5时，等效值约为50μM。在游离钙离子浓度约为1μM（与常见细胞内浓度相似）时，基本上所有活性都局限于pH7.0时的活性峰值。先前关于需要大量钙离子的报道可能反映了这样一个事实，即磷脂酰肌醇的钙缓冲能力意味着高底物浓度可以有效降低游离钙离子浓度。在低游离钙离子浓度下，能从与磷脂酰肌醇结合中置换出钙离子的阳离子可增强酶活性。完整细胞中的磷脂酰肌醇分解可能至少部分受细胞内游离钙离子浓度变化的控制。
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A membrane-bound activity catalysing phosphatidylinositol breakdown to 1,2-diacylglycerol, D-myoinositol 1:2-cyclic phosphate an D-myoinositol 1-phosphate. Properties and subcellular distribution in rat cerebral cortex.一种膜结合活性，催化磷脂酰肌醇分解为1,2 - 二酰基甘油、D - 肌醇1:2 - 环磷酸酯和D - 肌醇1 - 磷酸酯。大鼠大脑皮层中的性质和亚细胞分布。
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The hydrolysis of triphosphoinositide by a phosphodiesterase in rat kidney cortex.大鼠肾皮质中磷酸二酯酶对三磷酸肌醇的水解作用。
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