兔肌肉L-甘油-3-磷酸脱氢酶辅酶结合特性的研究。
A study of the coenzyme-binding characteristics of rabbit muscle L-glycerol 3-phosphate dehydrogenase.
作者信息
Bentley P, Dickinson F M
出版信息
Biochem J. 1974 Oct;143(1):11-17. doi: 10.1042/bj1430011.
Abstract
- The binding of NAD(+) and NADH to glycerol 3-phosphate dehydrogenase was studied in the pH range 6.0-9.0 at 25 degrees C and in the temperature range 16-43 degrees C at pH7.0. 2. The second-order velocity constants for the combination of NADH with the enzyme in the pH range 6.0-9.0 and for the combination of NAD(+) with the enzyme at pH6.0 were determined. 3. The velocity constant for the dissociation of the enzyme-NAD(+) complex at pH6.0 was measured.
摘要
- 在25摄氏度下，于pH值6.0 - 9.0范围内研究了NAD(+)和NADH与甘油3 - 磷酸脱氢酶的结合情况；在pH值7.0时，于16 - 43摄氏度温度范围内进行了研究。2. 测定了在pH值6.0 - 9.0范围内NADH与该酶结合的二级速度常数，以及在pH值6.0时NAD(+)与该酶结合的二级速度常数。3. 测量了在pH值6.0时酶 - NAD(+)复合物解离的速度常数。
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