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兔肌肉L-甘油-3-磷酸脱氢酶辅酶结合特性的研究。

A study of the coenzyme-binding characteristics of rabbit muscle L-glycerol 3-phosphate dehydrogenase.

作者信息

Bentley P, Dickinson F M

出版信息

Biochem J. 1974 Oct;143(1):11-17. doi: 10.1042/bj1430011.

DOI:10.1042/bj1430011
PMID:4377218
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168346/
Abstract
  1. The binding of NAD(+) and NADH to glycerol 3-phosphate dehydrogenase was studied in the pH range 6.0-9.0 at 25 degrees C and in the temperature range 16-43 degrees C at pH7.0. 2. The second-order velocity constants for the combination of NADH with the enzyme in the pH range 6.0-9.0 and for the combination of NAD(+) with the enzyme at pH6.0 were determined. 3. The velocity constant for the dissociation of the enzyme-NAD(+) complex at pH6.0 was measured.
摘要
  1. 在25摄氏度下，于pH值6.0 - 9.0范围内研究了NAD(+)和NADH与甘油3 - 磷酸脱氢酶的结合情况；在pH值7.0时，于16 - 43摄氏度温度范围内进行了研究。2. 测定了在pH值6.0 - 9.0范围内NADH与该酶结合的二级速度常数，以及在pH值6.0时NAD(+)与该酶结合的二级速度常数。3. 测量了在pH值6.0时酶 - NAD(+)复合物解离的速度常数。
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e16f/1168346/64f93893692a/biochemj00573-0030-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e16f/1168346/3d3ea331fbdb/biochemj00573-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e16f/1168346/130d479e8c55/biochemj00573-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e16f/1168346/64f93893692a/biochemj00573-0030-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e16f/1168346/3d3ea331fbdb/biochemj00573-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e16f/1168346/130d479e8c55/biochemj00573-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e16f/1168346/64f93893692a/biochemj00573-0030-b.jpg

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本文引用的文献

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J Biol Chem. 1963 Apr;238:1538-43.
2
Kinetic studies of liver alcohol dehydrogenase.肝脏乙醇脱氢酶的动力学研究
Biochem J. 1962 Aug;84(2):244-54. doi: 10.1042/bj0840244.
3
The nonprotein component of alpha-glycerophosphate dehydrogenase. Physical and chemical properties of the crystalline rabbit muscle enzyme.α-甘油磷酸脱氢酶的非蛋白质成分。结晶兔肌肉酶的物理和化学性质。
Human Glycerol 3-Phosphate Dehydrogenase: X-ray Crystal Structures That Guide the Interpretation of Mutagenesis Studies.
人甘油-3-磷酸脱氢酶：指导基因突变研究解释的 X 射线晶体结构。
Biochemistry. 2019 Feb 26;58(8):1061-1073. doi: 10.1021/acs.biochem.8b01103. Epub 2019 Jan 31.
4
Mapping metabolic changes by noninvasive, multiparametric, high-resolution imaging using endogenous contrast.利用内源性对比进行非侵入性、多参数、高分辨率成像来描绘代谢变化。
Sci Adv. 2018 Mar 7;4(3):eaap9302. doi: 10.1126/sciadv.aap9302. eCollection 2018 Mar.
5
A study of the kinetics and mechanism of rabbit muscle L-glycerol 3-phosphate dehydrogenase.兔肌肉L-3-磷酸甘油脱氢酶的动力学及作用机制研究
Biochem J. 1974 Oct;143(1):19-27. doi: 10.1042/bj1430019.
6
Estimation of rate and dissociation constants involving ternary complexes in reactions catalysed by yeast alcohol dehydrogenase.酵母乙醇脱氢酶催化反应中涉及三元复合物的速率常数和解离常数的估算。
Biochem J. 1978 Jun 1;171(3):629-37. doi: 10.1042/bj1710629.
J Biol Chem. 1959 Sep;234:2308-13.
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Biochem J. 1964 Apr;91(1):161-71. doi: 10.1042/bj0910161.
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Biochem J. 1973 Dec;135(4):853-9. doi: 10.1042/bj1350853.
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J Biol Chem. 1969 Mar 25;244(6):1547-51.