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兔肌肉L-甘油-3-磷酸脱氢酶的纯化及性质

Purification and properties of rabbit muscle L-glycerol 3-phosphate dehydrogenase.

作者信息

Bentley P, Dickinson F M, Jones I G

出版信息

Biochem J. 1973 Dec;135(4):853-9. doi: 10.1042/bj1350853.

Abstract

A modified procedure has been developed for the purification of rabbit muscle l-glycerol 3-phosphate dehydrogenase. The product of the preparation satisfies all criteria of homogeneity. Some physical properties of the enzyme have been re-investigated. The results suggest that previous preparations may have been contaminated with significant amounts of heavy-molecular-weight protein.

摘要

已开发出一种改良方法用于纯化兔肌肉L-甘油-3-磷酸脱氢酶。制备得到的产物满足所有均一性标准。对该酶的一些物理性质进行了重新研究。结果表明,之前的制备物可能已被大量高分子量蛋白质污染。

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