兔肌肉L-3-磷酸甘油脱氢酶的动力学及作用机制研究
A study of the kinetics and mechanism of rabbit muscle L-glycerol 3-phosphate dehydrogenase.
作者信息
Bentley P, Dickinson F M
出版信息
Biochem J. 1974 Oct;143(1):19-27. doi: 10.1042/bj1430019.
Abstract
- The kinetics of oxidation of l-glycerol 3-phosphate by NAD(+) and of reduction of dihydroxyacetone phosphate by NADH catalysed by rabbit muscle glycerol 3-phosphate dehydrogenase were studied over the range pH6-9. 2. The enzyme was found to catalyse the oxidation of glyoxylate by NAD(+) at pH8.0 and the kinetics of this reaction were also studied. 3. The results are consistent with a compulsory mechanism of catalysis for glycerol 3-phosphate oxidation and dihydroxyacetone phosphate reduction in the intermediate regions of pH, but modifications to the basic mechanism are required to fully explain results at the extremes of the pH range, with these substrates and for glyoxylate oxidation at pH8.0.
摘要
- 研究了兔肌甘油3-磷酸脱氢酶催化的NAD(+)氧化l-甘油3-磷酸以及NADH还原磷酸二羟丙酮在pH6 - 9范围内的动力学。2. 发现在pH8.0时该酶催化NAD(+)氧化乙醛酸，并对该反应的动力学进行了研究。3. 结果表明，在pH的中间区域，甘油3-磷酸氧化和磷酸二羟丙酮还原的催化机制为强制机制，但对于这些底物在pH范围两端的结果以及pH8.0时乙醛酸氧化的结果，需要对基本机制进行修正才能完全解释。
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