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1
A study of the kinetics and mechanism of rabbit muscle L-glycerol 3-phosphate dehydrogenase.兔肌肉L-3-磷酸甘油脱氢酶的动力学及作用机制研究
Biochem J. 1974 Oct;143(1):19-27. doi: 10.1042/bj1430019.
2
A study of the coenzyme-binding characteristics of rabbit muscle L-glycerol 3-phosphate dehydrogenase.兔肌肉L-甘油-3-磷酸脱氢酶辅酶结合特性的研究。
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3
Effect of NADH-X on cytosolic glycerol-3-phosphate dehydrogenase.NADH-X对胞质甘油-3-磷酸脱氢酶的影响。
Arch Biochem Biophys. 1998 Dec 15;360(2):195-205. doi: 10.1006/abbi.1998.0939.
4
Dihydroxyacetone sulfate as substrate analogue for alpha-glycerol phosphate dehydrogenase.二羟基丙酮硫酸酯作为α-甘油磷酸脱氢酶的底物类似物。
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5
1-Halo analogs of dihydroxyacetone 3-phosphate. The effects of the fluoro analog on cytosolic glycerol-3-phosphate dehydrogenase and triosephosphate isomerase.3-磷酸二羟基丙酮的1-卤代类似物。氟代类似物对胞质甘油-3-磷酸脱氢酶和磷酸丙糖异构酶的影响。
Biochemistry. 1975 May 20;14(10):2252-8. doi: 10.1021/bi00681a033.
6
Ability of cytosolic malate dehydrogenase and lactate dehydrogenase to increase the ratio of NADPH to NADH oxidation by cytosolic glycerol-3-phosphate dehydrogenase.胞质苹果酸脱氢酶和乳酸脱氢酶通过胞质甘油-3-磷酸脱氢酶提高NADPH与NADH氧化比率的能力。
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7
Reexamination of the kinetics of the transfer of NADH between its complexes with glycerol-3-phosphate dehydrogenase and with lactate dehydrogenase.重新审视烟酰胺腺嘌呤二核苷酸（NADH）在其与甘油-3-磷酸脱氢酶复合物以及与乳酸脱氢酶复合物之间转移的动力学。
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8
Rabbit muscle L-glycerol-3-phosphate dehydrogenase. Substrate activity of 3,4-dihydroxybutyl 1-phosphonate and 4-hydroxy-3-oxobutyl 1-phosphonate.兔肌肉L-甘油-3-磷酸脱氢酶。3,4-二羟基丁基1-膦酸酯和4-羟基-3-氧代丁基1-膦酸酯的底物活性。
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9
Direct transfer of NADH between alpha-glycerol phosphate dehydrogenase and lactate dehydrogenase: fact or misinterpretation?α-甘油磷酸脱氢酶与乳酸脱氢酶之间NADH的直接转移：事实还是误解？
Proc Natl Acad Sci U S A. 1989 Sep;86(17):6464-8. doi: 10.1073/pnas.86.17.6464.
10
Purification and characterization of glycerol-3-phosphate dehydrogenase of Saccharomyces cerevisiae.酿酒酵母3-磷酸甘油脱氢酶的纯化与特性分析
FEBS Lett. 1992 Aug 17;308(2):130-2. doi: 10.1016/0014-5793(92)81259-o.

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Glycerol 3-Phosphate Dehydrogenase Catalyzed Hydride Transfer: Enzyme Activation by Cofactor Pieces.甘油3-磷酸脱氢酶催化的氢化物转移：辅因子片段对酶的激活作用。
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2
Glycerol 3-Phosphate Dehydrogenase: Role of the Protein Conformational Change in Activation of a Readily Reversible Enzyme-Catalyzed Hydride Transfer Reaction.甘油-3-磷酸脱氢酶：在易于可逆的酶催化氢转移反应中蛋白质构象变化的作用。
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6
Primary Deuterium Kinetic Isotope Effects: A Probe for the Origin of the Rate Acceleration for Hydride Transfer Catalyzed by Glycerol-3-Phosphate Dehydrogenase.初级氘动力学同位素效应：甘油-3-磷酸脱氢酶催化氢化物转移速率加速起源的探针
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7
Enzyme architecture: optimization of transition state stabilization from a cation-phosphodianion pair.酶的结构：阳离子 - 磷酸二阴离子对实现过渡态稳定化的优化
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A substrate in pieces: allosteric activation of glycerol 3-phosphate dehydrogenase (NAD+) by phosphite dianion.呈碎片状的底物：亚磷酸二阴离子对3-磷酸甘油脱氢酶（NAD⁺）的变构激活作用
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9
A study of the coenzyme-binding characteristics of rabbit muscle L-glycerol 3-phosphate dehydrogenase.兔肌肉L-甘油-3-磷酸脱氢酶辅酶结合特性的研究。
Biochem J. 1974 Oct;143(1):11-17. doi: 10.1042/bj1430011.
10
Substrate channeling in glycolysis: a phantom phenomenon.糖酵解中的底物通道化：一种虚拟现象。
Proc Natl Acad Sci U S A. 1991 Jan 15;88(2):497-501. doi: 10.1073/pnas.88.2.497.

本文引用的文献

1
Studies on the mechanism of enzyme-catalyzed oxidation reduction reactions. IV. A proposed mechanism for the over-all reaction catalyzed by liver alcohol dehydrogenase.酶催化氧化还原反应机制的研究。IV. 肝脏乙醇脱氢酶催化总反应的一种 proposed 机制。 注：“proposed”在这里直接保留英文，因为不太明确其准确的中文释义在该语境下最合适的表述，需结合更专业的知识进一步确定，比如“推测的”“提出的”等。
Biochemistry. 1962 Jan;1:47-52. doi: 10.1021/bi00907a008.
2
The purification of nicotinamide adenine dinucleotide and kinetic effects of nucleotide impurities.烟酰胺腺嘌呤二核苷酸的纯化及核苷酸杂质的动力学效应
J Biol Chem. 1963 Apr;238:1538-43.
3
Some observations on the preparation and properties of dihydronicotinamide-adenine dinucleotide.关于二氢烟酰胺腺嘌呤二核苷酸制备及性质的一些观察
Biochem J. 1962 Aug;84(2):240-4. doi: 10.1042/bj0840240.
4
Kinetic studies of liver alcohol dehydrogenase.肝脏乙醇脱氢酶的动力学研究
Biochem J. 1962 Aug;84(2):244-54. doi: 10.1042/bj0840244.
5
Some physical and chemical properties of crystalline alpha-glycerophosphate dehydrogenase.
Arch Biochem Biophys. 1958 May;75(1):125-41. doi: 10.1016/0003-9861(58)90403-x.
6
The free-energy changes for the reduction of diphosphopyridine nucleotide and the dehydrogenation of L-malate and L-glycerol 1-phosphate.二磷酸吡啶核苷酸还原以及L-苹果酸和L-磷酸甘油脱氢反应的自由能变化。
Biochem J. 1953 Apr;54(1):86-94. doi: 10.1042/bj0540086.
7
Structural studies on rabbit muscle glycerol 3-phosphate dehydrogenase and a comparison of chemical and physical determinations of its molecular weight.
J Biol Chem. 1969 Mar 25;244(6):1631-44.
8
Purification and properties of rabbit muscle L-glycerol 3-phosphate dehydrogenase.兔肌肉L-甘油-3-磷酸脱氢酶的纯化及性质
Biochem J. 1973 Dec;135(4):853-9. doi: 10.1042/bj1350853.
9
Protein fluorescence of nicotinamide nucleotide-dependent dehydrogenases.烟酰胺核苷酸依赖性脱氢酶的蛋白质荧光
Biochem J. 1972 Jul;128(4):933-40. doi: 10.1042/bj1280933.
10
A study of the coenzyme-binding characteristics of rabbit muscle L-glycerol 3-phosphate dehydrogenase.兔肌肉L-甘油-3-磷酸脱氢酶辅酶结合特性的研究。
Biochem J. 1974 Oct;143(1):11-17. doi: 10.1042/bj1430011.

兔肌肉L-3-磷酸甘油脱氢酶的动力学及作用机制研究

A study of the kinetics and mechanism of rabbit muscle L-glycerol 3-phosphate dehydrogenase.

作者信息

Bentley P, Dickinson F M

出版信息

Biochem J. 1974 Oct;143(1):19-27. doi: 10.1042/bj1430019.

DOI:10.1042/bj1430019
PMID:4377219
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1168347/
Abstract
  1. The kinetics of oxidation of l-glycerol 3-phosphate by NAD(+) and of reduction of dihydroxyacetone phosphate by NADH catalysed by rabbit muscle glycerol 3-phosphate dehydrogenase were studied over the range pH6-9. 2. The enzyme was found to catalyse the oxidation of glyoxylate by NAD(+) at pH8.0 and the kinetics of this reaction were also studied. 3. The results are consistent with a compulsory mechanism of catalysis for glycerol 3-phosphate oxidation and dihydroxyacetone phosphate reduction in the intermediate regions of pH, but modifications to the basic mechanism are required to fully explain results at the extremes of the pH range, with these substrates and for glyoxylate oxidation at pH8.0.
摘要
  1. 研究了兔肌甘油3-磷酸脱氢酶催化的NAD(+)氧化l-甘油3-磷酸以及NADH还原磷酸二羟丙酮在pH6 - 9范围内的动力学。2. 发现在pH8.0时该酶催化NAD(+)氧化乙醛酸，并对该反应的动力学进行了研究。3. 结果表明，在pH的中间区域，甘油3-磷酸氧化和磷酸二羟丙酮还原的催化机制为强制机制，但对于这些底物在pH范围两端的结果以及pH8.0时乙醛酸氧化的结果，需要对基本机制进行修正才能完全解释。