Tada T, Okumura K, Takemori T, Arai T
Allerg Immunol (Leipz). 1974;20-21(4):427-34.
Immunogenicity of culture filtrates of various air-born fungi with respect to reaginic antibody formation was tested in the rat. Animals immunized with two successive injections of culture filtrates of fungi with the aid of Bordetella pertussis vaccine as an adjuvant produced fairly high titers of reaginic antibodies of the IgE class. Little cross-reactivity was detected between reagins induced by different strains of fungi. The reaginic immunogenicity was only found in the culture fieltrate produced by the surface culture, in which large amounts of conidia were formed, but not in the filtrate obtained from the shaking culture, in which only mycelial components were growing. Therefore, it was suggested that allergens responsible for the reaginic antibody formation derive from the conidium but not from the mycelium. The results indicate that air-born fungi are potentially immunogenic for production of reaginic antibodies, and further suggest their causative roles in human atopic diseases.
在大鼠中测试了各种空气传播真菌的培养滤液对于反应素抗体形成的免疫原性。借助百日咳博德特氏菌疫苗作为佐剂，用真菌培养滤液对动物进行两次连续注射后，产生了相当高滴度的IgE类反应素抗体。在不同真菌菌株诱导的反应素之间几乎未检测到交叉反应性。反应素免疫原性仅在表面培养产生的培养滤液中发现，其中形成了大量分生孢子，而在振荡培养获得的滤液中未发现，振荡培养中仅生长菌丝成分。因此，有人提出负责反应素抗体形成的变应原源自分生孢子而非菌丝体。结果表明，空气传播真菌对于反应素抗体的产生具有潜在免疫原性，并进一步表明它们在人类特应性疾病中的致病作用。
