通过在葡聚糖凝胶G - 200柱上进行凝胶过滤，从抗副流感病毒抗体中分离血凝抑制物。
The separation of haemagglutination inhibitors from antibodies to parainfluenza viruses by gel filtration on a Sephadex G-200 column.
作者信息
Mekler L B, Pichushkov A V, Zakstelskaya L J
出版信息
Immunology. 1967 Aug;13(2):219-21.
Abstract
Normal rabbit sera and sera of rabbits hyperimmunized against parainfluenza viruses types I and III were examined. Non-specific inhibitors of parainfluenza haemagglutinin were found in 19S and 4S fractions prepared by gel filtration. Specific antibodies were found in the 7S fraction and these could, therefore, be clearly separated from the non-specific inhibitors by gel filtration on a Sephadex G-200 column.
摘要
对正常兔血清以及用I型和III型副流感病毒进行超免疫的兔血清进行了检测。通过凝胶过滤制备的19S和4S组分中发现了副流感血凝素的非特异性抑制剂。在7S组分中发现了特异性抗体，因此，通过在葡聚糖凝胶G-200柱上进行凝胶过滤，可以将这些特异性抗体与非特异性抑制剂清楚地分离。
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