抗原结合诱导Fc构象改变：鸡免疫球蛋白中蛋白A反应位点的产生
Induction of an Fc conformational change by binding of antigen: the generation of protein A-reactive sites in chicken immunoglobulin.
作者信息
Barkas T, Watson C M
出版信息
Immunology. 1979 Mar;36(3):557-61.
Abstract
Chicken antibody alone does not bind to Staphylococcus aureus or protein A-Sepharose. Immune complexes containing chicken antibodies do bind, however, showing protein A-binding sites exist on the molecules. Binding does not occur via the antigen contained in the complex. Complexes formed in far antigen excess also bind, suggesting that an induced conformational change in the Fc region of the immunoglobulin occurs but only a fraction of the total chicken antibody is reactive.
摘要
单独的鸡抗体不与金黄色葡萄球菌或蛋白A-琼脂糖结合。然而，含有鸡抗体的免疫复合物确实能结合，这表明分子上存在蛋白A结合位点。结合不是通过复合物中所含的抗原发生的。在抗原大量过剩时形成的复合物也能结合，这表明免疫球蛋白Fc区域发生了诱导性构象变化，但只有一部分鸡抗体具有反应活性。
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