微小ColE1质粒pVH51的双向复制
Bidirectional replication of the mini-ColE1 plasmid pVH51.
作者信息
Eichenlaub R, Sparks R B, Helinski D R
出版信息
J Bacteriol. 1979 Apr;138(1):257-60. doi: 10.1128/jb.138.1.257-260.1979.
Abstract
Replicating molecules of the min-ColE1 plasmid pVH51 have been examined by electron microscopy after cleavage with the restriction endonuclease EcoRI. Replication apparently starts at a unique site indistinguishable from the origin of replication used by the parental plasmid ColE1. In contrast to ColE1, the structure of the majority of the replication intermediates was consistent with a bidirectional mode of replication. A minor portion of the molecules appeared to replicate unidirectionally in either direction from the same origin.
摘要
用限制性内切酶EcoRI切割后，通过电子显微镜对小ColE1质粒pVH51的复制分子进行了检查。复制显然起始于一个独特位点，该位点与亲本质粒ColE1所使用的复制起点无法区分。与ColE1不同，大多数复制中间体的结构与双向复制模式一致。一小部分分子似乎从同一原点向任一方向单向复制。
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