Abreu S L, Bancroft F C, Stewart W E
J Biol Chem. 1979 May 25;254(10):4114-8.
The effects of priming mouse cells with interferon on the production of interferon and its mRNA were investigated. Interferon-treated (primed) mouse L929 cells produce 3 to 10 times more interferon than do nonprimed cells following induction with Newcastle disease virus. Interferon appears 2 to 4 h sooner in the primed cultures than in nonprimed cultures and interferon production by primed cells becomes resistant to inhibition by actinomycin D about 4 h sooner than interferon production in nonprimed cells. Interferon mRNA is detected in primed-induced cells about 2 h earlier than in nonprimed-induced cells. It reaches peak levels about 2 to 4 earlier in primed cells, but it also disappears sooner in primed cells. The total amounts of interferon mRNA isolated from primed-induced cells and nonprimed-induced cells were indistinguishable, by the methods utilized. Therefore, although primed cells can produce significantly more interferon and make interferon mRNA sooner than nonprimed cells, the total amount of interferon mRNA produced is apparently not increased, nor is its half-life prolonged in primed cells. Thus, enhanced interferon production in primed cells may result from enhanced efficiency of translation of interferon mRNA in the primed cells.
研究了用干扰素预处理小鼠细胞对干扰素及其mRNA产生的影响。经干扰素处理（预处理）的小鼠L929细胞在用新城疫病毒诱导后产生的干扰素比未预处理的细胞多3至10倍。在预处理培养物中，干扰素出现的时间比未预处理培养物早2至4小时，并且预处理细胞产生干扰素比未预处理细胞对放线菌素D的抑制作用产生抗性的时间早约4小时。在预处理诱导的细胞中检测到干扰素mRNA的时间比未预处理诱导的细胞早约2小时。它在预处理细胞中达到峰值水平的时间早约2至4小时，但在预处理细胞中消失得也更快。通过所使用的方法，从预处理诱导细胞和未预处理诱导细胞中分离出的干扰素mRNA总量没有差异。因此，尽管预处理细胞比未预处理细胞能产生显著更多的干扰素且能更快地产生干扰素mRNA，但产生的干扰素mRNA总量显然没有增加，其半衰期在预处理细胞中也没有延长。因此，预处理细胞中干扰素产生的增强可能是由于预处理细胞中干扰素mRNA翻译效率的提高。
