一种用于检测抗流感抗体的微孔板免疫酶测定法。
A microplate immunoenzyme assay for anti-influenza antibodies.
作者信息
Lambre C, Kasturi K N
出版信息
J Immunol Methods. 1979;26(1):61-7. doi: 10.1016/0022-1759(79)90041-3.
An enzyme-linked immunosorbent assay (ELISA) using horseradish peroxidase is described for the detection and quantitation of anti-influenza virus antibodies. Compared with complement fixation and hemagglutination inhibition tests, ELISA is far superior with respect to sensitivity and reliability. Non-specific viral inhibitors present in sera do not affect the titer in ELISA. Its sensitivity, close to that of radioimmunoassay permits detection of small amounts of antibodies in pulmonary secretions and supernatants from in vitro spleen cell cultures.
描述了一种使用辣根过氧化物酶的酶联免疫吸附测定（ELISA）法，用于检测和定量抗流感病毒抗体。与补体结合试验和血凝抑制试验相比，ELISA在灵敏度和可靠性方面具有显著优势。血清中存在的非特异性病毒抑制剂不会影响ELISA中的滴度。其灵敏度接近放射免疫测定，能够检测肺分泌物和体外脾细胞培养上清液中的少量抗体。
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