Paul W E, Benacerraf B, Siskind G W, Goidl D A, Reisfeld R A
J Exp Med. 1969 Jul 1;130(1):77-89. doi: 10.1084/jem.130.1.77.
Rabbits immunized with killed type III pneumococci respond to anamnestic challenge by type specific polysaccharide (S III) with the synthesis of anti-S III antibody if a long interval is allowed to elapse between primary and secondary immunization. A study of the anti-S III antibody produced early and late in the immune response revealed no change in molecular class, banding pattern of dissociated light chains, or S III binding characteristics as measured under equilibrium conditions or by study of dissociation kinetics utilizing radioiodinated p-OH-benzyl-S III. Sequential booster injections of S III into rabbits primarily immunized with whole organisms 8 or 9 months earlier led to a progressive decrease in the number of animals showing successful anamnestic responses and in the magnitude of those responses. It is concluded that S III depletes the antigen sensitive cell population in the secondary response largely because of its limited ability to stimulate sustained proliferation by such cells.
用灭活的Ⅲ型肺炎球菌免疫的兔子，如果在初次免疫和二次免疫之间间隔较长时间，对特异性多糖（SⅢ）的回忆性攻击会通过合成抗SⅢ抗体作出反应。对免疫反应早期和晚期产生的抗SⅢ抗体的研究表明，在平衡条件下测量或通过利用放射性碘标记的对羟基苄基-SⅢ研究解离动力学时，分子类别、解离轻链的条带模式或SⅢ结合特性均无变化。对8或9个月前用全菌初次免疫的兔子连续注射SⅢ进行加强免疫，导致出现成功回忆反应的动物数量以及这些反应的强度逐渐下降。得出的结论是，SⅢ在二次反应中耗尽了抗原敏感细胞群体，主要是因为它刺激这些细胞持续增殖的能力有限。
