伯纳特柯克斯体的生物化学：6-磷酸葡萄糖酸脱氢酶
Biochemistry of Coxiella burnetii: 6-phosphogluconic acid dehydrogenase.
作者信息
McDonald T L, Mallavia L
出版信息
J Bacteriol. 1970 Apr;102(1):1-5. doi: 10.1128/jb.102.1.1-5.1970.
Abstract
Purified preparations of the rickettsial agent, Coxiella burnetii, have been examined for their ability to decarboxylate 6-phosphogluconate. The enzyme 6-phosphogluconic acid dehydrogenase [6-phospho-d-gluconate: NADP (nicotinamide adenine dinucleotide phosphate) oxidoreductase (decarboxylating), EC 1.1.1.44] was detected in extracts, but not in whole-cell preparations of C. burnetii. Both extracts and whole cells were shown to be free from contaminating host enzyme activity. Partial characterization of the enzyme has shown that it is substrate-dependent, specific for NADP, and requires magnesium for activity. The pH optimum of the rickettsial enzyme is 8.0.
摘要
已对纯化的立克次氏体病原体——伯氏考克斯氏体的制剂进行检测，以确定其使6-磷酸葡萄糖酸脱羧的能力。在提取物中检测到了6-磷酸葡萄糖酸脱氢酶[6-磷酸-D-葡萄糖酸：NADP（烟酰胺腺嘌呤二核苷酸磷酸）氧化还原酶（脱羧），EC 1.1.1.44]，但在伯氏考克斯氏体的全细胞制剂中未检测到。提取物和全细胞均显示没有宿主酶活性的污染。对该酶的部分特性分析表明，它依赖于底物，对NADP具有特异性，并且活性需要镁。立克次氏体酶的最适pH值为8.0。
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本文引用的文献
1
Oxidation of phosphohexonate and pentose phosphoric acids by yeast enzymes: Oxidation of phosphohexonate. II. Oxidation of pentose phosphoric acids.酵母酶对磷酸己糖和戊糖磷酸的氧化作用：磷酸己糖的氧化。II. 戊糖磷酸的氧化。
Biochem J. 1938 Sep;32(9):1626-44. doi: 10.1042/bj0321626.
2
INCORPORATION OF ACETATE-1-C INTO LIPID BY TYPHUS RICKETTSIAE.斑疹伤寒立克次体将乙酸-1-C掺入脂质的过程。
J Bacteriol. 1960 Oct;80(4):508-12. doi: 10.1128/jb.80.4.508-512.1960.
3
Formation of pentose phosphate from 6-phosphogluconate.由6-磷酸葡萄糖酸形成戊糖磷酸。
Science. 1950 May 19;111(2890):543-4. doi: 10.1126/science.111.2890.543.
4
Phosphogluconic acid dehydrogenase from yeast.酵母磷酸葡萄糖酸脱氢酶
J Biol Chem. 1951 Nov;193(1):371-81.
5
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
6
Crystalline D-gluconate 6-phosphate dehydrogenase.结晶态6-磷酸-D-葡萄糖酸脱氢酶
J Biol Chem. 1961 Nov;236:2975-80.
7
Studies on the physiology of rickettsiae. III. Glucose phosphorylation and hexokinase activity in Coxiella burnetii.立克次氏体生理学研究。III. 贝氏柯克斯体中的葡萄糖磷酸化作用和己糖激酶活性。
J Bacteriol. 1962 Mar;83(3):538-43. doi: 10.1128/jb.83.3.538-543.1962.
8
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9
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J Bacteriol. 1962 Jan;83(1):206-7. doi: 10.1128/jb.83.1.206-207.1962.
10
The effect of tumours, of leukaemia, and of some viruses associated with them, on the plasma lactic dehydrogenase activity of mice.肿瘤、白血病以及与它们相关的一些病毒对小鼠血浆乳酸脱氢酶活性的影响。
Br J Cancer. 1961 Dec;15(4):860-7. doi: 10.1038/bjc.1961.100.
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