立克次氏体生理学研究。V. 伯纳特柯克斯体对氨基甲酰磷酸的代谢
STUDIES ON THE PHYSIOLOGY OF RICKETTSIAE. V. METABOLISM OF CARBAMYL PHOSPHATE BY COXIELLA BURNETII.
作者信息
MALLAVIA L, PARETSKY D
出版信息
J Bacteriol. 1963 Aug;86(2):232-8. doi: 10.1128/jb.86.2.232-238.1963.
Abstract
Mallavia, L. (University of Kansas, Lawrence) and D. Paretsky. Studies on the physiology of rickettsiae. V. Metabolism of carbamyl phosphate by Coxiella burnetii. J. Bacteriol. 86:232-238. 1963.-Preparations of disrupted Coxiella burnetii catalyze synthesis of citrulline from ornithine and carbamyl phosphate at an optimal pH of 7.0 to 7.5. Rickettsial synthesis of the pyrimidine precursor, ureidosuccinate, is demonstrated and confirmed by isolating C(14)-labeled ureidosuccinate from reaction mixtures of carbamyl phosphate and labeled aspartate. The data suggest a further rickettsial synthesis of orotate and imply rickettsial competence for host-independent pyrimidine synthesis.
摘要
马拉维亚，L.（堪萨斯大学，劳伦斯）和D.帕雷茨基。立克次氏体生理学研究。V.伯氏考克斯氏体对氨基甲酰磷酸的代谢。《细菌学杂志》86:232 - 238。1963年。——破碎的伯氏考克斯氏体制剂在最适pH值7.0至7.5的条件下催化从鸟氨酸和氨基甲酰磷酸合成瓜氨酸。通过从氨基甲酰磷酸和标记天冬氨酸的反应混合物中分离出C(14)标记的脲基琥珀酸，证实了立克次氏体嘧啶前体脲基琥珀酸的合成。数据表明立克次氏体进一步合成乳清酸，并暗示立克次氏体具有独立于宿主进行嘧啶合成的能力。
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