通过亲和层析对肝癌组织培养细胞中合成酪氨酸转氨酶的核糖体进行部分纯化。
Partial purification by affinity chromatography of tyrosine aminotransferase-synthesizing ribosomes from hepatoma tissue culture cells.
作者信息
Miller J V, Cuatrecasas P, Thompson E B
出版信息
Proc Natl Acad Sci U S A. 1971 May;68(5):1014-8. doi: 10.1073/pnas.68.5.1014.
Abstract
This report describes a cell-free protein-synthesizing system derived from hepatoma tissue culture cells in which the synthesis of tyrosine aminotransferase is enhanced in relation to total protein synthesis. The enhancement was achieved by using affinity chromatography to select a specific fraction of ribosomes in which the proportion of tyrosine aminotransferase synthesis is higher than it is in unfractionated ribosomes.
摘要
本报告描述了一种源自肝癌组织培养细胞的无细胞蛋白质合成系统，在该系统中，酪氨酸转氨酶的合成相对于总蛋白质合成有所增强。这种增强是通过使用亲和色谱法来选择核糖体的特定部分实现的，在该部分中，酪氨酸转氨酶合成的比例高于未分级核糖体中的比例。
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2
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J Biol Chem. 1965 Jan;240:322-31.
3
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5
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