从菜花芽线粒体中分离出一种“苹果酸”酶及其性质

Biochem J. 1971 May;122(4):495-501. doi: 10.1042/bj1220495.

A ;malic' enzyme [l-malate-NAD oxidoreductase (decarboxylating), EC 1.1.1.39] has been isolated from cauliflower bud mitochondria and partially purified. 2. The enzyme is specific for l-malate and has an absolute requirement for either Mn(2+), Co(2+) or Mg(2+). 3. The enzyme shows activity with both NAD(+) and NADP(+), but NAD(+) is the preferred cofactor. 4. No appreciable oxaloacetate decarboxylase activity is present in the enzyme preparations even at low pH values. 5. The enzyme is inhibited by NADH and by oxaloacetate and stimulated by SO(4) (2-) and by low concentrations of CoA. 6. The regulatory properties of the enzyme support the proposed role of the enzyme in the utilization of tricarboxylic acid-cycle acids for energy production when glycolysis is suppressed.

苹果酸酶[L-苹果酸-NAD氧化还原酶（脱羧），EC 1.1.1.39]已从花椰菜芽线粒体中分离出来并进行了部分纯化。2. 该酶对L-苹果酸具有特异性，对Mn(2+)、Co(2+)或Mg(2+)有绝对需求。3. 该酶对NAD(+)和NADP(+)均有活性，但NAD(+)是首选的辅因子。4. 即使在低pH值下，酶制剂中也没有明显的草酰乙酸脱羧酶活性。5. 该酶受到NADH和草酰乙酸的抑制，并受到SO(4) (2-)和低浓度辅酶A的刺激。6. 该酶的调节特性支持了该酶在糖酵解受抑制时利用三羧酸循环酸进行能量产生的 proposed role（此处原文可能有误，暂按字面翻译为“提出的作用”）。

相似文献

Isolation and properties of a 'malic' enzyme from cauliflower bud mitochondria.

Biochem J. 1971 May;122(4):495-501. doi: 10.1042/bj1220495.

Properties and regulation of leaf nicotinamide-adenine dinucleotide phosphate-malate dehydrogenase and 'malic' enzyme in plants with the C4-dicarboxylic acid pathway of photosynthesis.

Biochem J. 1970 Sep;119(2):273-80. doi: 10.1042/bj1190273.

Activation of NAD-linked malic enzyme in intact plant mitochondria by exogenous coenzyme A.

Arch Biochem Biophys. 1984 May 15;231(1):233-42. doi: 10.1016/0003-9861(84)90383-7.

Isolation, properties and role in progesterone biosynthesis of cytosolic malic enzyme from human term placenta.

Placenta. 1987 Mar-Apr;8(2):175-84. doi: 10.1016/0143-4004(87)90020-8.

Nicotinamide-adenine dinucleotide-linked "malic" enzyme in flight muscle of the tse-tse fly (Glossina) and other insects.

Biochem J. 1976 Nov 15;160(2):253-62. doi: 10.1042/bj1600253.

Identification of an NAD(P)+-dependent 'malic' enzyme in small-intestinal-mucosal mitochondria.

Biochem J. 1979 Oct 15;184(1):185-8. doi: 10.1042/bj1840185.

The mitochondrial malic enzymes. I. Submitochondrial localization and purification and properties of the NAD(P)+-dependent enzyme from adrenal cortex.

J Biol Chem. 1975 Aug 10;250(15):5877-84.

Oxalacetate decarboxylase and pyruvate carboxylase activities, and effect of sulfhydryl reagents in malic enzyme from Sulfolobus solfataricus.

Biochim Biophys Acta. 1988 Nov 23;957(2):301-11. doi: 10.1016/0167-4838(88)90287-7.

Regulation of 'malic' enzyme of Solanum tuberosum by metabolites.

Biochem J. 1974 Jan;137(1):45-53. doi: 10.1042/bj1370045.

[Study of properties of NADP malate dehydrogenase from corn leaves].

Biokhimiia. 1976 Jul;41(6):1014-22.

引用本文的文献

The Role of Low-Molecular-Weight Organic Acids in Metal Homeostasis in Plants.

Int J Mol Sci. 2024 Sep 2;25(17):9542. doi: 10.3390/ijms25179542.

The versatility of plant organic acid metabolism in leaves is underpinned by mitochondrial malate-citrate exchange.

Plant Cell. 2021 Dec 3;33(12):3700-3720. doi: 10.1093/plcell/koab223.

The control of NAD specific malic enzyme from cauliflower bud mitochondria by metabolites.

Planta. 1975 Jan;126(3):197-211. doi: 10.1007/BF00388963.

Oligomeric enzymes in the C4 pathway of photosynthesis.

Photosynth Res. 1990 Dec;26(3):161-70. doi: 10.1007/BF00033129.

Properties and physiological function of a glutathione reductase purified from spinach leaves by affinity chromatography.

Planta. 1978 Jan;139(1):9-17. doi: 10.1007/BF00390803.

Effect of temperature on the pathways of NADH-oxidation in broad-bean mitochondria.

Planta. 1979 Jan;144(4):359-65. doi: 10.1007/BF00391579.

Two forms of NADP-dependent malic enzyme in expanding maize leaves.

Planta. 1979 Jan;144(3):283-9. doi: 10.1007/BF00388771.

A possible plasma membrane particle containing malic enzyme activity.

Planta. 1981 May;151(6):506-11. doi: 10.1007/BF00387427.

Divalent metal ions in plant mitochondria and their role in interactions with proteins and oxidative stress-induced damage to respiratory function.

Plant Physiol. 2010 Feb;152(2):747-61. doi: 10.1104/pp.109.147942. Epub 2009 Dec 14.

Comparison of the Kinetic Behavior toward Pyridine Nucleotides of NAD-Linked Dehydrogenases from Plant Mitochondria.

Plant Physiol. 1990 Sep;94(1):189-93. doi: 10.1104/pp.94.1.189.

本文引用的文献

Protein measurement with the Folin phenol reagent.

J Biol Chem. 1951 Nov;193(1):265-75.

Biosynthesis of dicarboxylic acids by carbon dioxide fixation. III. Enzymatic synthesis of iota-malic acid by reductive carboxylation of pyruvic acid.

J Biol Chem. 1950 Dec;187(2):863-74.

KINETICS OF REGULATORY ENZYMES. KINETIC ORDER OF THE YEAST DIPHOSPHOPYRIDINE NUCLEOTIDE ISOCITRATE DEHYDROGENASE REACTION AND A MODEL FOR THE REACTION.

J Biol Chem. 1965 Jun;240:2682-90.

Pyruvate carboxylation and plant metabolism.

Biol Rev Camb Philos Soc. 1962 May;37:215-56. doi: 10.1111/j.1469-185x.1962.tb01611.x.

Induction of malic enzyme and of oxalacetate decarboxylase in three lactic acid bacteria.

J Gen Microbiol. 1961 Jul;25:415-20. doi: 10.1099/00221287-25-3-415.

The oxidative decarboxylation of malate by Ascaris lumbricoides.

J Biol Chem. 1957 Apr;225(2):921-33.

Malate utilization by a group D Streptococcus. II. Evidence for allosteric inhibition of an inducible malate dehydrogenase (decarboxylating) by ATP and glycolytic intermediate products.

Biochim Biophys Acta. 1969 Apr 22;178(2):205-12. doi: 10.1016/0005-2744(69)90390-8.

Mechanism of malate utilization in Ascaris-muscle mitochondria.

Eur J Biochem. 1970 Feb;12(3):540-3. doi: 10.1111/j.1432-1033.1970.tb00884.x.

Existence and properties of two malic enzymes in Escherichia coli especially of NAD-linked enzyme.

J Biochem. 1969 Sep;66(3):379-87. doi: 10.1093/oxfordjournals.jbchem.a129156.

The oxidation of malate by mitochondria isolated from cauliflower buds.

Eur J Biochem. 1970 Sep;16(1):96-102. doi: 10.1111/j.1432-1033.1970.tb01058.x.

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

Biochem J. 1971 May;122(4):495-501. doi: 10.1042/bj1220495.

A ;malic' enzyme [l-malate-NAD oxidoreductase (decarboxylating), EC 1.1.1.39] has been isolated from cauliflower bud mitochondria and partially purified. 2. The enzyme is specific for l-malate and has an absolute requirement for either Mn(2+), Co(2+) or Mg(2+). 3. The enzyme shows activity with both NAD(+) and NADP(+), but NAD(+) is the preferred cofactor. 4. No appreciable oxaloacetate decarboxylase activity is present in the enzyme preparations even at low pH values. 5. The enzyme is inhibited by NADH and by oxaloacetate and stimulated by SO(4) (2-) and by low concentrations of CoA. 6. The regulatory properties of the enzyme support the proposed role of the enzyme in the utilization of tricarboxylic acid-cycle acids for energy production when glycolysis is suppressed.

苹果酸酶[L-苹果酸-NAD氧化还原酶（脱羧），EC 1.1.1.39]已从花椰菜芽线粒体中分离出来并进行了部分纯化。2. 该酶对L-苹果酸具有特异性，对Mn(2+)、Co(2+)或Mg(2+)有绝对需求。3. 该酶对NAD(+)和NADP(+)均有活性，但NAD(+)是首选的辅因子。4. 即使在低pH值下，酶制剂中也没有明显的草酰乙酸脱羧酶活性。5. 该酶受到NADH和草酰乙酸的抑制，并受到SO(4) (2-)和低浓度辅酶A的刺激。6. 该酶的调节特性支持了该酶在糖酵解受抑制时利用三羧酸循环酸进行能量产生的 proposed role（此处原文可能有误，暂按字面翻译为“提出的作用”）。

Isolation and properties of a 'malic' enzyme from cauliflower bud mitochondria.

作者信息

出版信息

相似文献

引用本文的文献

本文引用的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

从菜花芽线粒体中分离出一种“苹果酸”酶及其性质

Isolation and properties of a 'malic' enzyme from cauliflower bud mitochondria.

作者信息

出版信息