Gaskin F, Clayton R B
J Lipid Res. 1972 Jan;13(1):106-14.
Earlier experiments have shown that significantly more endogenously generated NADPH is available for reduction of corticosterone in liver homogenates from C57BL/10 male mice than in those from the DBA/2 strain. To test the effect of this interstrain difference upon a representative NADPH-requiring biosynthetic pathway in vitro, the biosynthesis of cholesterol from mevalonic acid was studied in homogenates of livers from the two strains of mice, with and without addition of an NADPH-generating system. The incorporation of mevalonic acid into cholesterol in homogenates from the C57BL/10 strain is little affected by omission of the NADPH-generating system, but in the DBA/2 strain, addition of an NADPH-generating system is necessary to elevate the level of cholesterol synthesis to that of the C57BL/10 strain. Without this addition, the DBA/2 homogenate mainly produces lanosterol and other precursors of cholesterol which require NADPH for their further metabolism.
早期实验表明，与DBA/2品系的雄性C57BL/10小鼠肝脏匀浆相比，前者内源性生成的NADPH显著更多，可用于还原皮质酮。为了在体外测试这种品系间差异对一条典型的需要NADPH的生物合成途径的影响，研究了在添加和不添加NADPH生成系统的情况下，这两种品系小鼠肝脏匀浆中由甲羟戊酸合成胆固醇的过程。在C57BL/10品系的匀浆中，甲羟戊酸掺入胆固醇的过程几乎不受NADPH生成系统缺失的影响，但在DBA/2品系中，必须添加NADPH生成系统才能将胆固醇合成水平提高到C57BL/10品系的水平。如果不添加，DBA/2匀浆主要产生羊毛甾醇和其他胆固醇前体，它们的进一步代谢需要NADPH。
