Douch P G, Smith J N
Biochem J. 1971 Nov;125(2):385-93. doi: 10.1042/bj1250385.
The metabolism of m-tert.-butylphenyl N-methylcarbamate was studied in mice and five species of insects. Both the tert.-butyl group and the N-methyl group were hydroxylated. The major phenolic metabolite was m-(beta-hydroxy-tert.-butyl)phenol, which was identified by mass spectroscopy. Significant amounts of dihydroxy compounds were formed at a constant rate from the start of the enzymic oxidation process. The considerable species variation in the yields of the different types of oxidation products suggests that N-demethylation and oxidation of the tert.-butyl groups were catalysed by different enzymes. A microsomal NADPH-dependent enzyme also catalysed the splitting of the ester link in the insecticide.
对小鼠和五种昆虫体内间叔丁基苯基N-甲基氨基甲酸酯的代谢进行了研究。叔丁基和N-甲基均发生了羟基化。主要的酚类代谢产物是间(β-羟基叔丁基)苯酚,通过质谱法进行了鉴定。从酶促氧化过程开始,就以恒定速率形成了大量的二羟基化合物。不同类型氧化产物产率的显著种间差异表明,N-去甲基化和叔丁基的氧化是由不同的酶催化的。一种微粒体NADPH依赖性酶也催化了杀虫剂中酯键的断裂。