长期饥饿对大鼠肝脏溶酶体的影响。
Effect of long-term starvation on the rat liver lysosomes.
作者信息
Pokrovsky A A, Tashev T A, Krustev L P, Tutelyan V A, Kravchenko L V
出版信息
Int J Vitam Nutr Res. 1976;46(1):75-82.
The effect of 120- and 240-h starvation on rats hepatocytes ultrastructure and particularly the changes of the lysosomes were studied. Eelectronmicroscopically and cytochemically there have been observed diminution of the number of mitochondria and degranulation and vacuolzation of the ER. At the same time Golgi complex was hypertrophied and the number of lysosomes was much increased, mainly those of the autophagic type. Biochemically was shown, that the activity of some acid hydrolases (beta-glucosidase, alpha- and beta-galactosidases, beta-N-acetylglucosaminidase, beta-glucuronidase and arylsulphatases A and B) in the liver of starved rats was markedly expressed. The sedimentation properties of the lysosomes and the lysosomal membrane stability was damaged as well. The data received have been discussed in the light of the reconstructive role of lysosomes.
研究了120小时和240小时饥饿对大鼠肝细胞超微结构的影响，特别是溶酶体的变化。通过电子显微镜和细胞化学方法观察到，线粒体数量减少，内质网出现脱颗粒和空泡化。同时，高尔基体肥大，溶酶体数量显著增加，主要是自噬型溶酶体。生化分析表明，饥饿大鼠肝脏中一些酸性水解酶（β-葡萄糖苷酶、α-和β-半乳糖苷酶、β-N-乙酰氨基葡萄糖苷酶、β-葡萄糖醛酸酶以及芳基硫酸酯酶A和B）的活性明显增强。溶酶体的沉降特性和溶酶体膜稳定性也受到损害。根据溶酶体的重建作用对所得数据进行了讨论。
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