Robinson N
J Neurol Neurosurg Psychiatry. 1972 Dec;35(6):865-72. doi: 10.1136/jnnp.35.6.865.
Penetration of a microneedle and injection of 4 μl. saline into the neocortex of the 5 day old rat brain produced no changes in behaviour of the rats up to 21 days post-injection. Within 24 hours sections indicated that tissue damage was apparent only at the pia-arachnoid membrane and where fluid was released; elsewhere the needle pathway was identified by the enzyme response. The enzyme histochemistry showed a marked increase in glial cell activity of some phosphatases within 24 hours at the site of injury; the pia-arachnoid and outer limiting membrane also showed abnormally high phosphatase reactions. NADH(2)-diaphorase was the only dehydrogenase that was raised in some nerve and glial cells at 24 hours post-injection but other dehydrogenases, mainly LDH and SDH, showed changes at four days post-injection. The phosphatases and 5'-nucleotidase previously showing intense glial cell enzyme reactions appeared to reach peaks of activity at eight days, and at 16 days the onset of scarring was apparent. In the pia-arachnoid enzyme activity increased to 21 days. Some enzymes, particularly AChE and MAO, showed no alterations of note throughout.
将微针插入5日龄大鼠脑的新皮层并注入4微升生理盐水，在注射后长达21天内大鼠行为未出现变化。在24小时内的切片显示，组织损伤仅在软脑膜 - 蛛网膜以及液体释放部位明显；在其他部位，针道可通过酶反应识别。酶组织化学显示，损伤部位在24小时内一些磷酸酶的胶质细胞活性显著增加；软脑膜 - 蛛网膜和外界膜也显示出异常高的磷酸酶反应。NADH(2)-黄递酶是注射后24小时在一些神经和胶质细胞中升高的唯一脱氢酶，但其他脱氢酶，主要是LDH和SDH，在注射后四天出现变化。先前显示强烈胶质细胞酶反应的磷酸酶和5'-核苷酸酶似乎在八天达到活性峰值，在16天瘢痕形成开始明显。在软脑膜中，酶活性增加至21天。一些酶，特别是AChE和MAO，在整个过程中未显示出明显变化。
