Al-Ali S Y, Robinson N
Am J Pathol. 1982 Apr;107(1):51-8.
A stab wound was produced in the adult rat cerebral cortex, and the progress of enzyme cytochemistry of phagocytosis was studied over 450 days. Light- and electron-microscopic observations were made to establish the origin of high acid phosphatase activity commonly seen at the site of brain lesions. Cells with phagocytic potential became differentiated and activated by the presence of degenerating neurons. The Golgi-lysosomal system of the phagocytes became elaborated, as evidenced by thiamine pyrophosphatase and acid phosphatase activities, the synthesis of acid phosphatase was increased, and the enzyme then secreted into the digestive vacuoles containing dead cells to be digested. Progress of the digestive process resulted in the accumulation of large amounts of acid phosphatase reaction product within the digestive vacuoles. The results showed that the phagocytes were the only detectable source of increased acid phosphatase activity at the site of injury in the cerebral cortex. In contrast to the phagocytes, newly formed multi-nucleated giant cells exhibited weak acid phosphatase, and intense cytochrome oxidase activities, the difference between the two cells reflecting the functional characteristics of each.
在成年大鼠大脑皮层制造刺伤，并在450天内研究吞噬作用的酶细胞化学进展。进行光镜和电镜观察以确定脑损伤部位常见的高酸性磷酸酶活性的来源。具有吞噬潜力的细胞因退化神经元的存在而分化并被激活。吞噬细胞的高尔基-溶酶体系统变得精细，硫胺素焦磷酸酶和酸性磷酸酶活性证明了这一点，酸性磷酸酶的合成增加，然后该酶分泌到含有待消化死细胞的消化液泡中。消化过程的进展导致消化液泡内大量酸性磷酸酶反应产物的积累。结果表明，吞噬细胞是大脑皮层损伤部位酸性磷酸酶活性增加的唯一可检测来源。与吞噬细胞相比，新形成的多核巨细胞酸性磷酸酶活性较弱，细胞色素氧化酶活性较强，这两种细胞之间的差异反映了各自的功能特征。
